Functional status of bone marrow mesenchymal stem cells from osteoarthritis patients and disease development

doi:10.3969/j.issn.2095-4344.2015.10.004

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells are crucial for bone and cartilage development and regeneration at a cellular level. Insufficient quantity and functional impairment of bone marrow mesenchymal stem cells is widely considered to be one of osteoarthritis causes.

OBJECTIVE: To explore the relationship between the functional status of bone marrow mesenchymal stem cells and disease progression in osteoarthritis patients.

METHODS: Thirty patients with osteoarthritis were enrolled from July 2013 to October 2014, and divided into control, mild osteoarthritis, and severe osteoarthritis groups, with 10 cases in each group. 5 mL bone marrow from the femur or tibia was extracted from each patient to isolate and culture bone marrow mesenchymal stem cells. Proliferation ability of cells at passage 3 was detected using cell counting kit-8; toluidine blue staining was performed at 14 days after chondrogenic induction; real-time PCR was used to detect the mRNA expression of Aggrecan and Col2A1 in the control group after chondrogenic induction.

RESULTS AND CONCLUSION: After in vitro culture, bone marrow mesenchymal stem cells grew adherently in polygonal and fusiform shape with multiple processes at uniform size. The cytoplasm contained larger particles and the nuclei were ovoid. Most of cells were in cell division phase. The proliferation ability was strongest in the control group and weakest in the severe osteoarthritis group. Cells from the three groups were all at plateau phase after 1 week culture. At 14 days after chondrogenic induction, the cells were polygonal and quasi-circular, and purple metachromatic granules distributed outside of the cytoplasm. The expression of Aggrecan and Col2A1 in the control group displayed an overexpression trend. These findings indicate that the functional status of bone marrow mesenchymal stem cells from osteoarthritis patients is negatively correlated with the severity of disease, which can influence the disease progression in osteoarthritis patients.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Osteoarthritis, Bone Marrow, Mesenchymal Stem Cells, Chondrocytes

CLC Number:

R394.2

Wang Zhi-zhou, Xu Chao, Han Ya-jun, Tie Xiao-jia, Guo Hong-liang, Yilihamu Tuoheti, Wang Jun-feng, Yan Bin, Bi Xiao-juan, Guan Zhen-peng. Functional status of bone marrow mesenchymal stem cells from osteoarthritis patients and disease development[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(10): 1495-1500.

Figures/Tables 5

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