Construction of targeting apolipoprotein E-modified liposome-mediated HSVtk/ganciclovir system

doi:10.3969/j.issn.2095-4344.2015.05.023

Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (5): 788-793.doi: 10.3969/j.issn.2095-4344.2015.05.023

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Construction of targeting apolipoprotein E-modified liposome-mediated HSVtk/ganciclovir system

Zhang Yue-hong^{1, 2}, Gao Ran-peng^{1, 2}, Yu Bao-feng^{1, 2}, Wang Hui-zhen^{1, 2}, Zhang Dong^{1, 2}, Gong Tao^{1, 2}, Guo Rui^{1, 2}, Wang Hai-long^{1, 2}, Cheng Niu-liang^{1, 2}, Xie Jun^{1, 2}, Niu Kai³, Qin Qin³, Xu Jun³

1Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
2Key Laboratory of Cellular Physiology by Shanxi Province and State Ministry of Education, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
3Department of General Surgery, Affiliated Shanxi People’s Hospital, Shanxi Medical University, Taiyuan 030012, Shanxi Province, China

Revised:2014-10-30 Online:2015-01-30 Published:2015-03-02
Contact: Xie Jun, M.D., Professor, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China; Key Laboratory of Cellular Physiology by Shanxi Province and State Ministry of Education, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China; Xu Jun, M.D., Chief physician, Professor, Department of General Surgery, Affiliated Shanxi People’s Hospital, Shanxi Medical University, Taiyuan 030012, Shanxi Province, China
About author:Zhang Yue-hong, Senior experimentalist, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China; Key Laboratory of Cellular Physiology by Shanxi Province and State Ministry of Education, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
Supported by:
the National Natural Science Foundation of China, No. 30901821, 81172136; the Program for Outstanding Innovative Teams of Higher Learning Institutions of Shanxi Province in 2011, No. [2011]205

Abstract

Abstract:

BACKGROUND: Suicide gene system without selectivity not only kills cancer cells, but also has the same effect on normal cells, so the construction of targeting vector of gene therapy has become an urgent problem to be solved.
OBJECTIVE: To evaluate killing effects of apolipoprotein E modified liposomes (apoE-lipoplexes) mediated TK/ganciclovir suicide gene transfection on hepatocellular carcinoma Li-7 cells.
METHODS: The apoE-lipoplexes packed pAFP-TK-IRES2-EGFP eukaryotic expression plasmids were transfected into Li-7 cells. HSVtk stably expressing cell clone (Li-7-tk) was screened. Fluorescence microscopy was used to observe the expression of enhanced green fluorescent protein. HSVtk gene expression was detected using western blotting. MTT method was used to evaluate the killing effects of HSVtk/GCV system on Li-7 cells.
RESULTS AND CONCLUSION: Stable clone (Li-7-tk) was obtained through screening suicide gene transfected Li-7 cells. After HSVtk/ganciclovir system acting on Li-7 cells, cell apoptosis rate was significantly increased (P < 0.01). In alpha fetoprotein-positive hepatocellular carcinoma cell line Li-7, suicide gene vector expresses stably and effectively kills cancer cells.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: Liver Neoplasms, Liposomes, alpha-Fetoproteins, Green Fluorescent Proteins

CLC Number:

R318

Zhang Yue-hong, Gao Ran-peng, Yu Bao-feng, Wang Hui-zhen, Zhang Dong, Gong Tao, Guo Rui, Wang Hai-long, Cheng Niu-liang, Xie Jun, Niu Kai, Qin Qin, Xu Jun. Construction of targeting apolipoprotein E-modified liposome-mediated HSVtk/ganciclovir system[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(5): 788-793.

Figures/Tables 3

References

[1] Jemal A, Bray F, Center MM, et al. Global cancer statistics. CA Cancer J Clin. 2011;61(2):69-90.
[2] Alsina AE. Liver transplantation for hepatocellular carcinoma. Cancer Control. 2010;17:83-86.
[3] Ong HT, Tan PK, Wang SM, et al. The tumor suppressor function of LECT2 in human hepatocellular carcinoma makes it a potential therapeutic target. Cancer Gene Ther. 2011; 18(6): 399-406.
[4] Ahn BC, Ronald JA, Kim YI, et al. Potent, tumor-specific gene expression in an orthotopic hepatoma rat model using a Survivin-targeted, amplifiable adenoviral vector. Gene Ther. 2011;18(6):606-612.
[5] Zeng ZJ, Xiang SG, Xue WW, et al. The cell death and DNA damages caused by the Tet-On regulating HSV-tk/GCV suicide gene system in MCF-7 cells. Biomed Pharmacother. 2014. [Epub ahead of print]
[6] Xiao X, Jin R, Li J, et al. The Antitumor Effect of Suicide Gene Therapy Using Bifidobacterium infantis-mediated Herpes Simplex Virus Thymidine Kinase/Ganciclovir in a Nude Mice Model of Renal Cell Carcinoma. Urology. 2014;84(4):982. e15-20.
[7] Vile RG, Catleden S, Marshall J, et al. Generation of an anti-tumour immune response in a non-immunogenic tumour: HSVtk killing in vivo stimulates a mononuclear cell infiltrate and a Th1-like profile of intratumoural cytokine expression. Cancer. 1997;71(2):267-274.
[8] Beltinger C,Fulda S,Kammertoens T, et al. Mitochondrial amplification of death signals determines thymidine kinase/ganciclovir-triggered activation of apoptosis. Cancer Res. 2000;60(12):3212-3127.
[9] Yu BF, Wu J, Zhang Y, et al. Ultrasound-targeted HSVtk and Timp3 gene delivery for synergistically enhanced antitumor effects in hepatoma.Cancer Gene Ther. 2013;20(5):290-297.
[10] Beltinger C, Fulda S, Kammertoens T, et al. Herpes simplex virus thymidine kinase/ganciclovir-induced apoptosis in-volves ligand-independent death receptor aggregation and activation of caspases.Proc Natl Acad Sci USA. 1999; 96(15): 8699-8704.
[11] Zhou XL, Shi YL, Li X. Inhibitory effects of the ultrasound-targeted microbubble destruction-mediated herpes simplex virus-thymidine kinase/ganciclovir system on ovarian cancer in mice. Exp Ther Med. 2014;8(4):1159-1163.
[12] Zhu R, Chen D, Lin D, et al. Adenovirus vector-mediated herpes simplex virus-thymidine kinase gene/ganciclovir system exhibits anti-tumor effects in an orthotopic hepatocellular carcinoma model. Pharmazie. 2014;69(7): 547-552.
[13] 查锡良.生物化学[M]. 7版.北京:人民卫生出版社,2008: 153-154.
[14] Mahley RW, Huang Y, Weisgraber KH. Putting cholesterol in its place: apoE and reverse cholesterol transport. J Clin Invest. 2006;116(5):1226-1229.
[15] 程牛亮.生物化学[M].1版.北京:高等教育出版社,2005: 109.
[16] 秦琴,莱智勇,高然朋,等.载脂蛋白ApoE修饰脂质体对肝癌细胞的靶向转染[J].中华实验外科杂志,2014,31(8):1709-1711.
[17] Lai Z, Qin Q, Yu B, et al. Construction of plasmid vector pAFP-HSVtk-IRES2-EGFP and its effect on the cytotoxicity of ganciclovir to hepatocellular carcinoma. Chin Med J (Engl). 2014;127(12):2337-2341.
[18] Raki M, Hakkarainen T, Bauerschmitz GJ, et al. Utility of TK/GCV in the context of highly effective oncolysis mediated by a serotype 3 receptor targeted oncolytic adenovirus. Gene Ther. 2007;14(19):1380-1388.
[19] Inoue H, Shiraki K, Murata K, et al. Adenoviral-mediated transfer of p53 gene enhances TRAIL-induced apoptosis in human hepatocellular carcinoma cells. Int J Mol Med. 2004; 14(2):271-275.
[20] Sheng S, Zheng J, Cui S, et al. Complete remission of multiple lung metastases after ablation of hepatocellular carcinoma by transarterial infusion with the p53 gene. Anticancer Drugs. 2014. [Epub ahead of print]
[21] Sangro B, Mazzolini G, Ruiz J, et al. Phase I trial of intratumoral injection of an adenovirus encoding interleukin-12 for advanced digestive tumors. J Clin Oncol. 2004; 22(8):1389-1397.
[22] Bai FL, Yu YH, Tian H, et al. Genetically engineered Newcastle disease virus expressing interleukin-2 and TNF-related apoptosis-inducing ligand for cancer therapy. Cancer Biol Ther. 2014;15(9):1226-1238.
[23] Maeda S, Wada H, Naito Y, et al. Interferon-α Acts on the S/G2/M Phases to Induce Apoptosis in the G1 Phase of an IFNAR2-expressing Hepatocellular Carcinoma Cell Line. J Biol Chem. 2014;289(34):23786-23795.
[24] Asklund T, Appelskog IB, Ammerpohl O, et al. Gap junction-mediated bystander effect in primary cultures of human malignant gliomas with recombinant expression of the HSVtk gene. Exp Cell Res. 2003;284(2): 185-195.
[25] Chiu CC, Kang YL, Yang TH, et al. Ectopic expression of herpes simplex virus-thymidine kinase gene in human non-small cell lung cancer cells conferred caspase-activated apoptosis sensitized by ganciclovir. Int J Cancer. 2002;102(4): 328-333.
[26] Kurozumi K, Tamiya T, Ono Y, et al. Apoptosis induction with 5-fluorocytosine/cytosine deaminase gene therapy for human malignant glioma cells mediated by adenovirus. J Neurooncol. 2004;66(1-2):117-127.
[27] van der Linden RR, Haagmans BL, Mongiat-Artus P, et al. Virus specific immune responses after human neoadjuvant adenovirus-mediated suicide gene therapy for prostate cancer. Eur Urol. 2005;48(1):153-161.
[28] Agard C, Ligeza C, Dupas B, et al. Immune-dependent distant bystander effect after adenovirus-mediated suicide gene transfer in a rat model of liver colorectal metastasis. Cancer Gene Ther. 2001;8(2):128-136.
[29] Gérolami R, Uch R, Bréchot C, et al. Gene therapy of hepatocarcinoma: a long way from the concept to the therapeutical impact. Cancer Gene Ther. 2003;10(9):649-660.
[30] Park JW, Hong K, Kirpotin DB, et al. Anti-HER2 immunoliposomes: enhanced efficacy attributable to targeted delivery. Clin Cancer Res. 2002;8(4): 1172-1181.

Construction of targeting apolipoprotein E-modified liposome-mediated HSVtk/ganciclovir system

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