Feasibility of cuttlebone/platelet-rich plasma compound in the repair of rabbit’s cartilage injuries

doi:10.3969/j.issn.2095-4344.2015.03.005

Abstract

Abstract:

BACKGROUND: Platelet-rich plasma is a kind of autologous platelet concentrate that includes a large number of growth factors, and it can promote the regeneration of the tissue cells and matrix, and stimulate tissue repair, whereas it is easy to flow, and thus cannot be effectively fixed in the injured site.

OBJECTIVE: To observe the effect of cuttlebone/platelet-rich plasma compound to repair the rabbit cartilage.

METHODS: Twenty-one big-ear white rabbits were randomly divided into three groups. A model of osteochondral injury was made in the cartilage of the weight-bearing condyle of the rabbit right knee in the three groups, and respectively treated with implantation of cuttlebone/platelet-rich plasma compound in the experimental group, implantation of gelatin sponge/platelet-rich plasma compound in the control group, and nothing in the blank control group. The specimens were examined at 12 weeks postoperatively for gross observation and histological observation.

RESULTS AND CONCLUSION: At 12 weeks after surgery, in the experimental group, the cartilage defect region was reduced significantly, chondrocyte-like cells appeared and arranged in alignment, showing a columnar distribution that is very close to the normal cartilage; in the control group, the cartilage defect region was narrowed a little, cartilage matrix-like tissues were visible but irregular; the blank control group, the cartilage defect region was narrowed slightly, and only bone cells, bone matrix and blood vessels were seen with no presence of cartilage or cartilage-like tissues. The defect diameter and depth in the experimental group were lower than those in the other two groups at 12 weeks after surgery (P < 0.05). These findings suggest that the cuttlebone/platelet-rich plasma compound can promote the healing of cartilage defects.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: Platelet-Rich Plasma, Cartilage, Braces

CLC Number:

R318

Bi Heng, Wang Qing, Liu Tao, Yang Li-ding, Yao Xiao, Pu Hong-run, Ye Guo-yu, Xiang Jun-yi. Feasibility of cuttlebone/platelet-rich plasma compound in the repair of rabbit’s cartilage injuries[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(3): 352-357.

Figures/Tables 2

2.1 实验动物数量分析 21只兔均进入结果分析。 2.2 富血小板血浆采集结果鉴定在实验组及对照组中随机抽取富血小板血浆样本各3份，用自动血液细胞仪进行血小板计数及手工复核。测量方法：用血红蛋白吸管分别在装有全血的离心管及富血小板血浆的离心管中吸取样本0.5 mL，以血小板稀释液稀释至2 mL，混匀，先用全自动血液计数，再取1滴注入清洁计数池内放置10 min，高倍光镜下做人工计数，同时比对全血中血小板数。实验所得到的富血小板血浆中血小板浓度为 (1 258.33±216.40)×109 L-1，而全血中的血小板浓度为(255.33±47.07)×109 L-1 ，富血小板血浆中血小板浓度为全血的4倍以上。实验组与对照组富血小板血浆血小板计数比较差异无显著性意义(P > 0.05)。 2.3 海螵蛸支架观察大体观察为瓷白色，呈肉眼可见的多孔状外观，纹理清晰，质地轻，脆性较大，硬度较低。低倍镜下可见海螵蛸中有支柱样结构，支柱之间相互平行，即为大体见之清晰纹理，相邻支柱间是与之相交排列的小梁结构，壁较薄，支柱与小梁相间排列，构成规则的网格结构，孔隙率为85%-90%，平均孔径为0.2 mm×0.09 mm。高倍镜下可见支柱与小梁样结构所构成的壁龛样网格结构更为明显，支柱与小梁表面光滑并呈现出板层结构。等离子消毒后支架呈黄白色，保持原有的多孔结构和明显的纹理，等离子改性前后脆性和硬度变化不大。低倍镜下观察水产物时发现材料保持了其多孔网状结构，仍由网格样结构构成，原有的支柱、小梁等结构依然存在。将采用Aghaloo离心法获得的自体富血小板血浆液与海螵蛸体混合，室温下静置2 h，见海螵蛸可以吸收部分血浆液，逐渐被染成浅红色并逐渐发生凝血，富血小板血浆黏附在其表面及网格中。 2.4 各组动物骨缺损模型修复观察结果大体观察(图3)：实验组的软骨缺损区域明显缩小，已明显修复，与周围软骨面没有明显分界，修复组织和周围软骨组织相似，中心区域有很小的区域和周围软骨组织不同，考虑为未修复区域，缺损直径为(1.0±0.5) mm，深度为(1.0±0.5) mm。对照组的软骨缺损区域轻度缩小，可明显观察到造模痕迹，但范围减小，软骨造模表面有滑膜附着，清理去滑膜后可以见软骨缺损区域，周围软骨组织生长非常不均衡、不规则，中心缺损深度直径为(4.0±0.5) mm，缺损深度为(3.5±0.5) mm。空白对照组的软骨缺损区域有轻度缩小，但从软骨下骨组织到软骨面仍然存在明显的缺损，有滑膜组织长入附着，清理滑膜组织后可见造模缺损区域，周围软骨组织生长不规则，缺损直径为(4.0±0.3) mm，缺损深度为(5.5± 0.5) mm。实验组缺损直径及深度明显低于其他两组(P < 0.05)，对照组与空白对照组缺损直径及深度比较差异无显著性意义(P > 0.05)。"

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