Adipose-derived stem cells transfected with adenovirus carrying bone morphogenetic protein 14 for repair of articular cartilage injury

doi:10.3969/j.issn.2095-4344.2015.01.010

Abstract

Abstract:

BACKGROUND: The articular cartilage has weak self-repair ability, mainly due to its lack of trophoblast cells in blood vessels and slow cell metabolism. Current treatment methods cannot restore the original function of the cartilage tissue, and cartilage tissue engineering in recent years has garnered increasing attention.
OBJECTIVE: To observe the effect of adipose-derived stem cells transfected with bone morphogenetic protein 14 combined with type I collagen sponge scaffold on the repair of articular cartilage injury in the knee of rabbits.
METHODS: Adipose-derived stem cells were isolated and cultured from rabbit subcutaneous adipose tissue, and transfected with Ad-CMV-BMP-14-IRES-hrGFP-1. Type I collagen sponge scaffold with the transfected adipose-derived stem cells was used to repair articular cartilage injury in the knee of rabbits. Twelve weeks after operation, the articular tissue was taken for gross assessment and histological evaluation.
RESULTS AND CONCLUSION: The expressions of bone morphogenetic protein 14, type II collagen and Sox-9 were higher in cells transfected with bone morphogenetic protein 14 than untransfected ones. At 12 weeks after operation, adipose-derived stem cells transfected with bone morphogenetic protein 14 combined with type I collagen sponge scaffold had good repair effect on articular cartilage injuries, and the injured cartilage tissues were smooth and had good texture, color and integration junction; adipose-derived stem cells combined with type I collagen sponge scaffold could partially repair the injured cartilage tissues that had similar color and texture to normal tissues, and there was a remarkable boundary between the repaired tissue and normal cartilage tissue; simple type I collagen sponge scaffold was almost collapsed, and no hyaline cartilage tissue formed. These findings indicate that transfection of bone morphogenetic protein 14 can strengthen the ability of adipose-derived stem cells dramatically to repair cartilage injuries.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Adipose Tissue, Mesenchymal Stem Cells, Bone Morphogenetic Proteins, Tissue Engineering

CLC Number:

R394.2

Ma Hong-bin, Li Yun-xiang, Wang Ming-lun. Adipose-derived stem cells transfected with adenovirus carrying bone morphogenetic protein 14 for repair of articular cartilage injury[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(1): 54-60.

Figures/Tables 3

References

[1] Counsel PD, Bates D, Boyd R, et al. Cell therapy in joint disorders.Sports Health. 2015;7 (1): 27-37.
[2] Smith GD, Knutsen G, Richardson JB.A clinical review of cartilage repair techniques.J Bone Joint Surg Br. 2005;87(4): 445-449.
[3] Mithoefer K, Saris DB, Kon E,et al.Guidelines for the Design and Conduct of Clinical Studies in Knee Articular Cartilage Repair: International Cartilage Repair Society Recommendations Based on Current Scientific Evidence and Standards of Clinical Care. Cartilage.2011; 2(2):100-121.
[4] Kon E, Delcogliano M, Filardo G,et al.Orderly osteochondral regeneration in a sheep model using a novel nano-composite multilayered biomaterial.J Orthop Res. 2010;28(1):116-124.
[5] 袁虹,张解元,张荣明. BMP-14 转染脂肪来源干细胞后与软骨细胞共培养的实验研究[J].中国修复重建外科杂志,2012,27(3): 349-353.
[6] Vacanti CA.The history of tissue engineering.J Cell Mol Med. 2006;10(3):569-576.
[7] Lindroos B, Suuronen R, Miettinen S.The potential of adipose stem cells in regenerative medicine.Stem Cell Rev. 2011;7(2): 269-291.
[8] Vinardell T, Sheehy EJ, Buckley CT,et al.A comparison of the functionality and in vivo phenotypic stability of cartilaginous tissues engineered from different stem cell sources.Tissue Eng Part A. 2012;18(11-12):1161-1170.
[9] Patricia Zuk.Adipose-Derived Stem Cells in Tissue Regeneration: A Review. ISRN Stem Cells.2013;10 (11):372- 391.
[10] Merceron C, Portron S, Masson M,et al.The effect of two- and three-dimensional cell culture on the chondrogenic potential of human adipose-derived mesenchymal stem cells after subcutaneous transplantation with an injectable hydrogel.Cell Transplant. 2011;20(10):1575-1588.
[11] Colnot C.Cell sources for bone tissue engineering: insights from basic science.Tissue Eng Part B Rev. 2011;17(6):449- 457.
[12] Huey DJ, Hu JC, Athanasiou KA.Unlike bone, cartilage regeneration remains elusive.Science. 2012;338(6109): 917-921.
[13] Catherine Baugé, Nicolas Girard, Eva Lhuissier, et al. Regulation and Role of TGFβ Signaling Pathway in Aging and Osteoarthritis Joints.Aging Dis. 2014;5(6):394-405.

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