Fluorescent supermagnetic nanoparticles-labeled adipose-derived mesenchymal stem cells in the three-dimensional culture system

doi:10.3969/j.issn.2095-4344.2015.01.008

Abstract

Abstract:

BACKGROUND: Fluorescent magnetic nanoparticles with the properties of quantum dots and magnetic particles have good biocompatibility, and can label cells effectively through endocytosis.
OBJECTIVE: To validate the feasibility of fluorescent magnetic nanoparticles in labeling human adipose-derived mesenchymal stem cells.
METHODS: Healthy human adipose tissue was extracted and adipose-derived mesenchymal stem cells were isolated in vitro by type I collagenase digestion. Passage 6 cells were incubated with the fluorescent magnetic nanoparticles overnight. Prussian blue staining and laser scanning confocal microscope were used to observe labeled adipose-derived mesenchymal stem cells in vitro after co-culturing with fluorescent magnetic nanoparticles. The tracing effect of labeled adipose-derived mesenchymal stem cells in vivo was detected by fluorescence imaging system.
RESULTS AND CONCLUSION: Prussian blue staining showed that the fluorescent magnetic nanoparticles dispersed in the cytoplasm of adipose-derived mesenchymal stem cells in the form of blue particles. Under the laser scanning confocal microscope, the nuclei of adipose-derived mesenchymal stem cells were dyed blue by Hoechest33258, and the cytoplasm was dyed green. The fluorescence imaging results showed that labeled human adipose-derived mesenchymal stem cells had good imaging results. Therefore, as an efficient tracer, the fluorescent magnetic nanoparticles can label human adipose-derived stem cells in vitro and provide a new method for transplantation and transformation of adipose-derived mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Adipose Tissue, Mesenchymal Stem Cells, Biological Markers

CLC Number:

R394.2

Lv Pin-lei, Su Yue-han, Cui Da-xiang, Wang Zheng. Fluorescent supermagnetic nanoparticles-labeled adipose-derived mesenchymal stem cells in the three-dimensional culture system [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(1): 44-48.

Figures/Tables 4

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