Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (50): 8122-8129.doi: 10.3969/j.issn.2095-4344.2014.50.015

Previous Articles     Next Articles

Bone marrow mesenchymal stem cell transplantation in the treatment of rat radiation-induced lung injury

Zheng Kai, Zhao Xing-wang, Wu Wei-zhen, Tan Jian-ming, Yang Shun-liang   

  1. Organ Transplant Institute, Fuzhou General Hospital of Nanjing Military Region, Fuzhou 350025, Fujian Province, China
  • Received:2014-11-09 Online:2014-12-03 Published:2014-12-03
  • Contact: Tan Jian-ming, M.D., Chief physician, Organ Transplant Institute, Fuzhou General Hospital of Nanjing Military Region, Fuzhou 350025, Fujian Province, China
  • About author:Zheng Kai, M.D., Associate chief physician, Organ Transplant Institute, Fuzhou General Hospital of Nanjing Military Region, Fuzhou 350025, Fujian Province, China
  • Supported by:

    the Military Science and Technology Project during the Twelfth Five-Year Plan, No. BWS11J004; the Science and Technology Project of Nanjing Military Region, No. 10z031; the Scientific Innovative Platform Construction of Fujian Province, No. 2010Y2006

PDF

439

Abstract:

BACKGROUND: Except for corticosteroids and antibiotics, there is no effective treatment for radiation-induced lung injury. Recent studies have shown that bone marrow mesenchymal stem cells appear to have strong tissue repair ability.
OBJECTIVE: To observe the therapeutic effect of bone marrow mesenchymal stem cells on radiation-induced lung injury in rats, and then to preliminarily explore the mechanism of action.
METHODS: Bone marrow mesenchymal stem cells from male Sprague-Dawley rats were isolated, cultured and  
identified in vitro. Sixty male Sprague-Dawley rats were subject to chest irradiation to establish radiation-induced lung injury models. Then, the model rats were randomly divided into treatment group and control group, respectively treated with tail vein injection of 2×109/L bone marrow mesenchymal stem cells and the same volume of normal saline. After 1, 2, 4, 6 weeks of irradiation, relevant parameters were detected. 
RESULTS AND CONCLUSION: After 1, 2, 4 weeks of irradiation, the lung coefficient was significantly lower in the treatment group than the control group (P < 0.05). Under the microscope, less inflammatory exudation was from lung tissue in the treatment group, and the alveoli and alveolar wall structure were basically complete. The degree of fibrosis was significantly milder in the treatment group than the control group. Compared with the control group, the levels of serum transforming growth factor β1 and hydroxyproline were significantly lower in the treatment group (P < 0.05) at 2 weeks of irradiation, the activity of superoxide dismutase in lung tissue was significantly higher in the treatment group
(P < 0.05) at 2, 4, 6 weeks of irradiation, the content of malondialdehyde was significantly lower in the treatment group
(P < 0.05) at 4 and 6 weeks of irradiation, and the expression of pulmonary surfactant B was significantly higher in the treatment group at 6 weeks of irradiation. PCR results showed that Sry expressed in the lung, liver, pancreas, kidneys in varying degrees, especially obviously in the lung. These findings indicate that bone marrow mesenchymal stem cells can migrate into the radiation-injured lung tissue, to promote repair of radiation-induced lung injury. The treatment mechanism may be to lesser lung tissue fibrosis by inhibiting inflammation and antioxidation.


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程


全文链接：

Key words: bone marrow, mesenchymal stem cells, radiation injuries, lung injury

CLC Number: