Bone mesenchymal stem cell sheet transplantation combined with autologous iliac bone for repair of alveolar cleft

doi:10.3969/j.issn.2095-4344.2014.50.013

Abstract

Abstract:

BACKGROUND: Autogenous iliac bone graft for repair of alveolar cleft often cannot achieve a stable therapeutic effect, and the graft resorption rate varies with each individual that is influenced by many factors.
OBJECTIVE: To observe the bone resorption after bone marrow mesenchymal stem cell sheet transplantation with autogenous iliac cancellous bone for alveolar cleft repair.
METHODS: Bilateral alveolar cleft models were prepared in dogs. Using the self-control method, bone marrow mesenchymal stem cell sheet combined with autogenous iliac bone (experimental group) and autogenous iliac bone alone (control group) were respectively implanted into the bilateral alveolar clefts. Mandible CT and microCT were used to compare the bone resorption rate, bone volume fraction, trabecular thickness, trabecular separation, and bone mineral density.
RESULTS AND CONCLUSION: The bone resorption rate was significantly lower in the experimental group than the control group at 3 and 6 postoperative months (P < 0.01); while, the bone volume fracture and bone mineral density of the experimental group were significantly higher than those in the control group at postoperative 6 months (P < 0.05), but the trabecular separation of the experimental group was significantly lower than that in the control group (P < 0.05). In addition, there was no difference in the trabecular thickness between the two groups (P > 0.05). These findings indicate that bone marrow mesenchymal stem cell sheet transplantation combined with autogenous iliac bone graft can reduce bone resorption rate and meanwhile, promote new bone formation.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cells, ilium, bone transplantation, transplantation, autologous, maxillofacial abnormalities

CLC Number:

R394.2

Shen Yue, Ma Hai-ying, Zhang Yan-sheng, Wang Juan, Shi Bing-zheng. Bone mesenchymal stem cell sheet transplantation combined with autologous iliac bone for repair of alveolar cleft[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(50): 8108-8112.

Figures/Tables 2

References

[1] 杨超,石冰. 牙槽突裂植骨术的技术改进与初步评价[J]. 中国实用口腔科杂志, 2012,5(6):327-332.
[2] Francis CS, Mobin SS, Lypka MA,et al.rhBMP-2 with a demineralized bone matrix scaffold versus autologous iliac crest bone graft for alveolar cleft reconstruction.Plast Reconstr Surg. 2013;131(5):1107-1115.
[3] Anker CJ, Holdridge SP, Baird B, et al. Ultraporous beta-tricalcium phosphate is well incorporated in small cavitary defects. Clin Orthop Relat Res. 2005;(434):251-257.
[4] Feichtinger M, Mossbock R, Karcher H. Evaluation of bone volume following bone grafting in patients with unilateral clefts of lip, alveolus and palate using a CT- guided three-dimensional navigation system. J Craniomaxillofac Surg, 2006; 34(3):144-149.
[5] Yang J, Yamato M, Shimizu T,et al.Reconstruction of functional tissues with cell sheet engineering.Biomaterials. 2007;28(34):5033-5043.
[6] Li HW, Sun JY.Effects of dicalcium silicate coating ionic dissolution products on human mesenchymal stem-cell proliferation and osteogenic differentiation.J Int Med Res. 2011;39(1):112-128.
[7] Han D, Li J, Guan X.Ectopic osteogenesis of hBMP-2 gene-transduced human bone mesenchymal stem cells/BCB.Connect Tissue Res. 2010;51(4):274-281.
[8] Li G, Wang X, Cao J,et al.Coculture of peripheral blood CD34+ cell and mesenchymal stem cell sheets increase the formation of bone in calvarial critical-size defects in rabbits.Br J Oral Maxillofac Surg. 2014;52(2):134-139.
[9] Zhou Y, Chen F, Ho ST,et al.Combined marrow stromal cell-sheet techniques and high-strength biodegradable composite scaffolds for engineered functional bone grafts. Biomaterials. 2007;28(5):814-824.
[10] Matsui K, Matsui A, Handa T,et al.Bone regeneration by octacalcium phosphate collagen composites in a dog alveolar cleft model.Int J Oral Maxillofac Surg. 2010;39(12): 1218-1225.
[11] Mafi P, Hindocha S, Mafi R, et al. Adult mesenchymal stem cells and cell surface characterization-a systematic review of the literature. Open Orthop J. 2011;5(Suppl 2):253-260.
[12] Sharma S, Schneider LF, Barr J,et al.Comparison of minimally invasive versus conventional open harvesting techniques for iliac bone graft in secondary alveolar cleft patients.Plast Reconstr Surg. 2011;128(2):485-491.
[13] 唐世杰,石伦刚.牙槽突裂植骨吸收的原因与对策[J]. 中国实用口腔科杂志,2012,5(6):332-336.
[14] Toloue SM, Chesnoiu-Matei I, Blanchard SB.A clinical and histomorphometric study of calcium sulfate compared with freeze-dried bone allograft for alveolar ridge preservation.J Periodontol. 2012;83(7):847-855.
[15] Egami M, Haraguchi Y, Shimizu T,et al. Latest status of the clinical and industrial applications of cell sheet engineering and regenerative medicine.Arch Pharm Res. 2014;37(1): 96-106.
[16] Chen T, Wang Y, Bu L,et al.An animal experiment of construction of functional tissue-engineered bone with cell sheet technology.Hua Xi Kou Qiang Yi Xue Za Zhi. 2011; 29(4):442-445.
[17] Kawase T, Okuda K, Kogami H, et al. Characterization of human cultured periosteal sheets expressing bone-forming potential: in vitro and in vivo animal studies. Tissue Eng Regen Med. 2009;3(3):218-229.
[18] Viateau V, Guillemin G, Bousson V,et al.Long-bone critical-size defects treated with tissue-engineered grafts: a study on sheep.J Orthop Res. 2007;25(6):741-749