In vitro osteogenic induction of bone marrow mesenchymal stem cells after co-transfection with Ad-hBMP-2 and Ad-hTGF-β1

doi:10.3969/j.issn.2095-4344.2014.50.001

Abstract

Abstract:

BACKGROUND: Advances in recombinant DNA technology provide a huge impetus to the development of bone tissue engineering research, which used for tissue-engineered bone construction can significantly improve the quality and efficiency of bone repair.
OBJECTIVE: To evaluate the in vitro osteogenic induction of Ad-hBMP-2 and Ad-hTGF-β1 co-transfected bone marrow mesenchymal stem cells and to elucidate their relationship in bone formation．
METHODS: Bone marrow mesenchymal stem cells from Sprague-Dawley rats were infected by high-titer recombinant adenoviruses carrying transforming growth factor-β1 and bone morphogenetic protein-2 genes, and then transfected cells were induced by exogenous genes detected with RT-PCR, ELISA, MTT and alkaline phosphatase test．
RESULTS AND CONCLUSION: Adenovirus-infected bone marrow mesenchymal stem cells could express exogenous genes detected by RT-PCR. MTT test and alkaline phosphatase test showed the proliferation ability of co-transfected bone marrow mesenchymal stem cells was the strongest and the alkaline phosphatase activity was the highest. It has been observed that bone marrow mesenchymal stem cells co-transfected with Ad-hBMP-2 and Ad-hTGF-β1 can be induced to form bone tissue cooperatively and synergistically.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cells, transforming growth factor beta1, bone
morphogenetic proteins

CLC Number:

R394.2

Chen Jian-ping, Qiu Jun-qin, Zeng Zhao-xun, Chen Zong-xiong, Yin Cheng-hui. In vitro osteogenic induction of bone marrow mesenchymal stem cells after co-transfection with Ad-hBMP-2 and Ad-hTGF-β1[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(50): 8037-8041.

Figures/Tables 2

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