Mechanism of ultrasound-mediated microbubbles enhancing homing effect of transplanted mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.45.015

Abstract

Abstract:

BACKGROUND: Animal studies have indicated ultrasound-mediated microbubbles can significantly enhance the effect of stem cell transplantation to treat ischemic diseases. But its mechanism is still unknown.

OBJECTIVE: To explore the mechanism of ultrasound-mediated microbubbles to significantly enhance the effect of stem cell transplantation in the treatment of ischemic diseases.

METHODS: Bone marrow mesenchymal stem cells and vascular endothelial cells of rats were cultured in vitro, and then randomized to three groups: control group with no intervention, ultrasound group exposed to ultrasound at 1 MHz, 1 W/cm² for 90 seconds, and ultrasound-mediated microbubble group treated with 5 μL liposomes ultrasound microbubbles containing fluorocarbon gases (about 2×10¹¹/L) and ultrasound exposure at 1 MHz,

1 W/cm² for 90 seconds.

RESULTS AND CONCLUSION: Compared to the control group, ultrasound-mediated microbubbles significantly increased expressions of vascular endothelial growth factor and stromal cell-derived factor 1 in the supernatant of vascular endothelial cells (P < 0.05); ultrasound had no effect on the expression of vascular endothelial growth factor, but decreased the level of stromal cell-derived factor 1 (P < 0.01). Ultrasound-mediated microbubbles and the ultrasound alone could significantly enhance the CXCR4 gene expression in bone marrow mesenchymal stem cells as compared with the control group (P < 0.01), but there was no difference between the ultrasound-mediated microbubble group and the ultrasound group (P > 0.05). These findings suggest that 1 W/cm² ultrasound-mediated microbubbles can promote vascular endothelial growth factor and stromal cell-derived factor 1 secretion by vascular endothelia cells, and meanwhile promote CXCR4 gene expression in bone marrow mesenchymal stem cells. This may be the mechanism of the ultrasound-mediated microbubbles enhancing homing effect of transplanted stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: sonication, endothelial cells, mesenchymal stem cells, liposomes

CLC Number:

R394.2

Qian Jian, Chen Fei, Fan Guo-feng, Sha Du-juan, Wang Lu-na, Li Qi-ming, Ma Hao, Chen Yi-bing, Zhang Jun. Mechanism of ultrasound-mediated microbubbles enhancing homing effect of transplanted mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(45): 7294-7298.

Figures/Tables 2

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