Rat’s Schwann cells induce neural differentiation of human umbilical cord mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2014.45.007

Abstract

Abstract:

BACKGROUND: Mesenchymal stem cells can differentiate into nerve cells by chemical induction or co-culture method, but whether mesenchymal stem cells co-cultured with Schwann cells differentiate into neuronal-like cells or Schwann-like cells is still controversial.

OBJECTIVE: To explore the inductive role of Schwann cells derived from rats in the differentiation of human umbilical cord mesenchymal stem cells.

METHODS: Cocultures of human umbilical cord mesenchymal stem cells (1×10⁹/L) and Schwann cells (1×10⁹/L) from neonatal rats were performed using transwell culture dishes. After 2 weeks of cocultures, morphology of the cultured human umbilical cord mesenchymal stem cells was observed, and the phenotypic changes of cells were also detected with immumocytochemistry techniques.

RESULTS AND CONCLUSION: After 2 weeks of cocultures, some differentiated cells showed neuron-like morphology, and expressed nestin, NF-200 and β-III-tubulin, but did not express Schwann cell special marker S100 and oligodendrocytes special marker MAB1580. These findings indicate that human umbilical cord mesenchymal stem cells can transdifferentiate into neuronal-like cells by cocultures with rat’s Schwann cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: umbilical cord, mesenchymal stem cells, Schwann cells, cell culture techniques

CLC Number:

R394.2

Zhu Jia-xue, Tao Hai-rong, Shen Zun-li. Rat’s Schwann cells induce neural differentiation of human umbilical cord mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(45): 7250-7254.

Figures/Tables 2

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