Expression of Toll-like receptor 2, Toll-like receptor 4 and Toll-like receptor 6 in peripheral blood mononuclear cells and their relationship with Treg immune response in children with Henoch-Schonlein purpura

doi:10.3969/j.issn.2095-4344.2014.38.027

Abstract

Abstract:

BACKGROUND: The influence of Toll-like receptor 2 (TLR2), Toll-like receptor 4 (TLR4), Toll-like receptor 6 (TLR6) signal transduction pathway and active Treg in children with Henoch-Schonlein purpura has been unknown.
OBJECTIVE: To investigate the expression of TLR2, TLR4 and TLR6 in peripheral blood mononuclear cells and Treg immune response in patients with Henoch-Schonlein purpura, and to explore the role of TLR2, TLR4, TLR6 and Treg activation in the pathogenesis of Henoch-Schonlein purpura.
METHODS: Forty-two hospitalized children with Henoch-Schonlein purpura were enrolled in this study. Another 15 healthy children were selected as controls. TLR2, TLR4 and TLR6 at protein level in peripheral blood mononuclear cells were detected by flow cytometey; reverse-transcription PCR and real-time PCR were used to evaluate the level of MyD88; the levels of transforming growth factor-β and interleukin-10 were measured by enzyme-linked immunosorbent assay. t-test or t’-test was used to compare the levels of these genes and proteins. Pearson’s correlation test was done for correlation analysis.
RESULTS AND CONCLUSION: Compared with the control group, the protein levels of TLR2, TLR4, TLR6 and the relative expression level of MyD88 mRNA were significantly up-regulated (P < 0.01). The serum levels of transforming growth factor-β and interleukin-10 were higher in the Henoch-Schonlein purpura children than the healthy children (P < 0.05). There was a significant correlation between the protein levels of TLR2, TLR4, TLR6 and mRNA level of MyD88 (P < 0.01), but no relationship was found between TLRs and interleukin-10, transforming growth factor-β (P > 0.05). The excessive activation of TLR2, TLR4, TLR6 may be involved in the process of Henoch-Schonlein purpura via MyD88-dependent pathway, and the compensatory activation of Treg may participate in protective immunity.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: purpura, child, Toll-like receptors, T-lymphocytes

CLC Number:

R318

Gao Hui-xiang, Tian Ling-ling, Chang Hong, Zhu Hai-yan, Zhang Qiu-ye. Expression of Toll-like receptor 2, Toll-like receptor 4 and Toll-like receptor 6 in peripheral blood mononuclear cells and their relationship with Treg immune response in children with Henoch-Schonlein purpura[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(38): 6222-6227.

Figures/Tables 2

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