Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (38): 6099-6104.doi: 10.3969/j.issn.2095-4344.2014.38.006
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Shi Jian-jie, Luo Zhi-bin, Chen Wen-xiong
Received:
2014-08-21
Online:
2014-09-10
Published:
2014-09-10
Contact:
Luo Zhi-bin, Master, Attending physician, Department of Stomatology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, China
About author:
Shi Jian-jie, Chief physician, Department of Stomatology, the Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, Guangdong Province, China
Supported by:
the Science and Technology Development Program of Guangdong Province, No. 2010B060900087
CLC Number:
Shi Jian-jie, Luo Zhi-bin, Chen Wen-xiong . Bone morphogenetic protein-4 compounded with platelet-rich plasma promotes bone healing[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(38): 6099-6104.
2.1 实验动物数量分析 实验选用大白兔24只,分为4组,实验过程无脱失,全部进入结果分析。 2.2 兔骨缺损区大体观察 大体标本可见12周时空白对照组成骨较差,缺损区中间凹陷,表面纤维组织增生;余各组成骨作用好,成骨饱满,表面光滑(图2)。 2.3 兔骨缺损区组织学观察 4周:富血小板血浆组、富血小板血浆+骨形态发生蛋白4组两组骨缺损处见新生血管丰富,其中富血小板血浆+骨形态发生蛋白4组可见较多的纤维性骨痂形成,局部见较多成骨细胞及软骨细胞增生活跃,骨移植颗粒开始降解,可见与骨干平行的骨样组织,富血小板血浆组见纤维组织生长,材料降解为网状,周围有炎性细胞和少量骨母细胞(见图3)。空白对照组、β-磷酸三钙+Bio-gide生物膜组两组骨缺损区见少量新生血管,β-磷酸三钙+Bio-gide生物膜组周可见大量散在骨粉颗粒及纤维组织结构,其间散在数量不等的成骨细胞和软骨细胞,成骨作用较对照组强。 8周:富血小板血浆+骨形态发生蛋白4组缺损区内骨粉颗粒明显较β-磷酸三钙+Bio-gide生物膜组、富血小板血浆组减少,伴随着大量骨样组织形成,钙化、沉积明显;β-磷酸三钙+Bio-gide生物膜组、富血小板血浆组局部见新生骨组织,少量破骨细胞,少量钙盐沉积,两组间差异无显著性意义;空白对照组出现以纤维性骨痂为主,自体骨边缘见少量新生骨组织,明显较β-磷酸三钙+Bio-gide生物膜组、富血小板血浆组少(图4)。 12周:富血小板血浆+骨形态发生蛋白4组主要为成熟骨,为板层骨,可见哈佛氏小管形成,散在少量编织骨,骨粉颗粒明显减少,少量破骨细胞(图5)。 2.4 组织学图像分析缺损区新生骨结果 计算机图像分析显示,伴随愈合时间推移,新骨形成逐渐增多,β-磷酸三钙颗粒逐渐降解,其中富血小板血浆+骨形态发生蛋白4组12周时新生骨略高于8周但两者差异无显著性意义(P > 0.05)。 术后4 周,富血小板血浆+骨形态发生蛋白4组新生骨略多于富血小板血浆组,但两组间差异无显著性意义 (P > 0.05) ,富血小板血浆组、富血小板血浆+骨形态发生蛋白4组两组骨缺损区新生骨以及新生血管均多于β-磷酸三钙+Bio-gide组实验侧(P < 0.01),β-磷酸三钙+Bio-gide组新成骨多于空白对照组(P < 0.01);术后4,8,12周,富血小板血浆+骨形态发生蛋白4组骨缺损区新生骨均多于β-磷酸三钙+Bio-gide组、富血小板血浆组两组骨缺损区(P < 0.01),富血小板血浆组略多于β-磷酸三钙+Bio-gide组,但两者差异无显著性意义(P > 0.05),β-磷酸三钙+ Bio-gide组、富血小板血浆组两组均多于空白对照组(P < 0.01)。"
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