Human umbilical cord mesenchymal stem cells for repair of combined radiation-wound skin injury and tumorigenicity in vitro

doi:10.3969/j.issn.2095-4344.2014.37.016

Abstract

Abstract:

BACKGROUND: Many scholars have experimentally confirmed the obvious effect of mesenchymal stem cells to repair radiation injury.
OBJECTIVE: To preliminarily investigate the mechanism of human umbilical cord mesenchymal stem cells promoting the healing of combined radiation-wound skin injury and whether they possess tumorigenicity in vitro.
METHODS: Fifteen Sprague-Dawley rats were randomly divided into three groups, five rats in each group. The right buttock of rats (2.5 cm×2.0 cm) was irradiated with 40 Gy β-rays produced by a linear accelerator, in which a round wound with a diameter of 1.5 cm was made. After 12 hours of modeling, human umbilical cord mesenchymal stem cells at three concentrations (5.0×10⁶, 1.0×10⁷ and 2.0×10⁷) were injected through tail vein of rats, and luciferin (20 mg/kg) was injected intraperitoneally. Cell distribution in vivo was traced using IVIS in vivo imaging system. The ability of human umbilical cord mesenchymal stem cells to form colonies was observed using the colony formation assay with soft agar.
RESULTS AND CONCLUSION: Human umbilical cord mesenchymal stem cells injected through tail vein of rats were mostly gathered in the lungs. Cells were accumulated in the injured site of rats injected with 2.0×10⁷ human umbilical cord mesenchymal stem cells; however, the fluorescence signal was not observed in the injured site of rats injected with 5.0×106 and 1.0×10⁷ human umbilical cord mesenchymal stem cells. The other results indicated human umbilical cord mesenchymal stem cells of low dose, medium dose and high dose had no colony formation on soft agar, but the tumor cells had a great ability to form colony. These findings indicate that human umbilical cord mesenchymal stem cells promote healing combined radiation-wound skin injury by local migration and exhibit no tumorigenicity in vitro in a short period.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: umbilical cord, mesenchymal stem cells, radiation injuries, carcinogenicity tests

CLC Number:

R394.2

Su Zhong-yi, Yang Zai-liang, Tang Yong-yong, Hu Jiang-wei, Sheng Hong-xia, Xu Man, Zhang Bin, Chen Hu. Human umbilical cord mesenchymal stem cells for repair of combined radiation-wound skin injury and tumorigenicity in vitro[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(37): 5993-5997.

Figures/Tables 1

References

[1] Grammaticos P, Giannoula E, Fountos GP.Acute radiation syndrome and chronic radiation syndrome.Hell J Nucl Med. 2013;16(1):56-59.
[2] 刘建功,郭万龙,张淑贤,等.间充质干细胞在急性辐射损伤治疗中的作用综述[J].辐射研究与辐射工艺学报,2010,28(3):134- 137.
[3] Dörr H, Meineke V.Acute radiation syndrome caused by accidental radiation exposure - therapeutic principles.BMC Med. 2011;9:126.
[4] Dainiak N.Rationale and recommendations for treatment of radiation injury with cytokines.Health Phys. 2010;98(6): 838-842.
[5] López M, Martín M.Medical management of the acute radiation syndrome.Rep Pract Oncol Radiother. 2011; 16(4): 138-146.
[6] Akita S.Treatment of Radiation Injury.Adv Wound Care (New Rochelle). 2014;3(1):1-11.
[7] Glover D, Harmer V.Radiotherapy-induced skin reactions: assessment and management.Br J Nurs. 2014;23(4):S28, S30-35.
[8] López M, Martín M.Medical management of the acute radiation syndrome.Rep Pract Oncol Radiother. 2011;16(4): 138-146.
[9] da Silva Meirelles L, Chagastelles PC, Nardi NB. Mesenchymal stem cells reside in virtually all post-natal organs and tissues.J Cell Sci. 2006;119(Pt 11):2204-2213.
[10] Baksh D, Yao R, Tuan RS.Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow.Stem Cells. 2007;25(6):1384-1392.
[11] 李丽春,何治,李剑平.胎盘间充质干细胞的体外诱导分化[J].中国组织工程研究,2013,17(6):1116-1121.
[12] Stimpfel M, Cerkovnik P, Novakovic S, et al.Putative mesenchymal stem cells isolated from adult human ovaries.J Assist Reprod Genet. 2014. [Epub ahead of print]
[13] Dominici M, Le Blanc K, Mueller I,et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006;8(4):315-317.
[14] 王芳,汤永红.间充质干细胞免疫原性和免疫调节作用的研究进展[J].中南医学科学杂志, 2014,42(1): 97-100.
[15] Lee M, Jeong SY, Ha J,et al. Low immunogenicity of allogeneic human umbilical cord blood-derived mesenchymal stem cells in vitro and in vivo.Biochem Biophys Res Commun. 2014;446(4):983-989.
[16] Newman RE, Yoo D, LeRoux MA,et al.Treatment of inflammatory diseases with mesenchymal stem cells.Inflamm Allergy Drug Targets. 2009;8(2):110-123.
[17] Linard C, Busson E, Holler V,et al.Repeated autologous bone marrow-derived mesenchymal stem cell injections improve radiation-induced proctitis in pigs.Stem Cells Transl Med. 2013; 2(11):916-927.
[18] Wang R, Yuan W, Zhao Q,et al.An experimental study of preventing and treating acute radioactive enteritis with human umbilical cord mesenchymal stem cells.Asian Pac J Trop Med. 2013;6(12):968-971.
[19] 朱红燕,张宏,傅晋翔,等.骨髓间充质干细胞与急性皮肤放射损伤的修复[J].中国组织工程研究与临床康复,2009,13(32):6303- 6308.
[20] Kotenko KB, Moroz BB, Nadezhina NM,et al.Mesenchymal stem cells transplantation in the treatment of radiation skin lesions.Patol Fiziol Eksp Ter. 2011;(1):20-25.
[21] Yang D, Sun S, Wang Z,et al.Stromal cell-derived factor-1 receptor CXCR4-overexpressing bone marrow mesenchymal stem cells accelerate wound healing by migrating into skin injury areas.Cell Reprogram. 2013;15(3):206-215.
[22] Fakhari S, Kalantar E, Nikzaban M,et al. Effect of Helicobacter pylori infection on stromal-derived factor-1/CXCR4 axis in bone marrow-derived mesenchymal stem cells.Adv Biomed Res. 2014;3:19.
[23] 张大庆.单核细胞趋化蛋白-1在骨髓间充质干细胞向胶质瘤细胞趋化中的作用研究[D]. 大连:大连医科大学,2012.
[24] 李兴泽,孟庆海,金澎,等.趋化因子CXCL-8趋化骨髓间充质干细胞迁移的体外效应[J]. 中国组织工程研究与临床康复, 2009, 13(10):1908-1912.
[25] 王楠,张利,李清刚,等.骨髓间充质干细胞治疗急性腹膜纤维化作用方式的探讨[J].解放军医学杂志,2011,36(12):1263-1268.
[26] 房林.骨髓间充质干细胞移植促进皮肤创伤愈合的实验研究[D].北京:北京协和医学院,2010.
[27] Maxson S, Lopez EA, Yoo D,et al.Concise review: role of mesenchymal stem cells in wound repair.Stem Cells Transl Med. 2012;1(2):142-149.
[28] Singer NG, Caplan AI.Mesenchymal stem cells: mechanisms of inflammation.Annu Rev Pathol. 2011;6:457-478.
[29] Krasnodembskaya A, Song Y, Fang X,et al. Antibacterial effect of human mesenchymal stem cells is mediated in part from secretion of the antimicrobial peptide LL-37.Stem Cells. 2010;28(12):2229-2238.
[30] Mei SH, Haitsma JJ, Dos Santos CC,et al.Mesenchymal stem cells reduce inflammation while enhancing bacterial clearance and improving survival in sepsis.Am J Respir Crit Care Med. 2010;182(8):1047-1057.
[31] Hocking AM, Gibran NS.Mesenchymal stem cells: paracrine signaling and differentiation during cutaneous wound repair. Exp Cell Res. 2010;316(14):2213-2219.
[32] Colwell AS, Beanes SR, Soo C,et al. Increased angiogenesis and expression of vascular endothelial growth factor during scarless repair.Plast Reconstr Surg. 2005;115(1):204-212.
[33] Smith AN, Willis E, Chan VT,et al. Mesenchymal stem cells induce dermal fibroblast responses to injury.Exp Cell Res. 2010;316(1):48-54.
[34] Ra JC, Shin IS, Kim SH,et al.Safety of intravenous infusion of human adipose tissue-derived mesenchymal stem cells in animals and humans.Stem Cells Dev. 2011;20(8):1297-1308.
[35] 秦秀军,张伟,李建国,等.人脂肪间充质干细胞的致瘤性研究[J].天津医药,2013,41(2):152-153.