Meropenem effects on biological characteristics of umbilical cord-derived mesenchymal stem cells during umbilical cord collection

doi:10.3969/j.issn.2095-4344.2014.37.007

Abstract

Abstract:

BACKGROUND: Due to the difficulty in the control of delivery and collection process, antibiotics are often added into the preservation fluid in order to avoid bacterial contamination but not affect cell growth and proliferation.
OBJECTIVE: To observe the effects of meropenem on the proliferation and differentiation potential of umbilical cord-derived mesenchymal stem cells.
METHODS: There were two groups in this experiment: control group, preservation fluid with penicillin- streptomycin (final concentration of 100 U/mL); experimental group, preservation fluid with meropenem (final mass concentration of 1.0 mg/L). 100 umbilical cord samples were collected in each group, and the positive rate was calculated. After isolation and culture, the passage 3 cells were used to draw a growth curve, flow cytometry analysis was used for phenotype determination, and osteogenic and adipogenic differentiation of cells were detected.
RESULTS AND CONCLUSION: The contamination rates were 3% (3/100) in the experimental group and 20% (20/100) in the control group, indicating that meropenem can obviously reduce the contamination rate. In the experimental group, the morphology, differentiation potential and cell phenotype of the passage 3 cells were all normal. The proliferation ability of cells showed no difference between the two groups. Therefore, meropenem can be added to the preservation fluid.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: umbilical cord, mesenchymal stem cells, beta-lactams, anti-bacterial agents

CLC Number:

R394.2

Liu Jun-jiang, Zhou Jian-yu, Huang Wen-jing, Hong Jing-xin . Meropenem effects on biological characteristics of umbilical cord-derived mesenchymal stem cells during umbilical cord collection[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(37): 5942-5946.

Figures/Tables 3

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