Reproductive effects of in vitro fertilization-embryo transfer: in vitro culture and cryopreservation of embryos

doi:10.3969/j.issn.2095-4344.2014.18.026

Abstract

Abstract:

BACKGROUND: Since the first successful birth of a "test tube baby", in vitro fertilization-embryo transfer technology has been greatly developed within 20 years.
OBJECTIVE: To retrospectively analyze the assisted reproductive effects of in vitro fertilization-embryo transfer, and to explore the culture of embryos in vitro and the application of cryopreservation technology.
METHODS: A total of 131 infertile patients received in vitro fertilization-embryo transfer or intracytoplasmic sperm injection in Department of Reproductive Medicine, Affiliated Hospital of Luzhou Medical College, China. There were 147 oocyte retrieval cycles and 124 transfer cycles, the mean age of the patients was 31.32 years. Standard long-protocol ovulation induction was given to 131 oocyte retrieval cycles, super-long-protocol ovulation induction was given to 4 oocyte retrieval cycles, and super-short-protocol ovulation induction was given to two oocyte retrieval cycles; 93 cases were transplanted with fresh embryos and 28 cases with frozen embryos. The clinical pregnancy rate, embryo implantation rate and abortion rate of patients receiving different ovulation induction protocols and fresh or frozen embryo transfer were observed.
RESULTS AND CONCLUSION: In the patients receiving standard long, super-long and super-short protocols for ovulation induction, the clinical pregnancy rate was respectively 34.7%, 50%, 20%; the embryo implantation rates were 19.1%, 22.2%, 30%; and abortion rate was 21.7%, 50%, 33.3%; in those patients, no case exhibited natural cycle of ovulation. In the patients receiving fresh embryo transfer, the clinical pregnancy rate was 33.6%, embryo implantation rate was 18.1%, and abortion rate was 20.1%; in frozen embryo transferred patients, the clinical pregnancy rate was 53.8%, embryo implantation rate was 22.3%, and abortion rate was 20.7%. Experimental findings indicate that, standard long-protocol ovulation induction has good effects, the efficacy of fresh embryo transfer is equivalent to that of frozen embryo transfer, in vitro culture and cryopreservation of the embryos are important steps in assisted reproductive technology and deserver further study.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: fertilization in vitro, embryo transfer, cryopreservation, pregnancy, reproductive technology, auxiliary

CLC Number:

R318

Wang Fang, Chen Shao-wei. Reproductive effects of in vitro fertilization-embryo transfer: in vitro culture and cryopreservation of embryos[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(18): 2939-2944.

Figures/Tables 3

References

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