Tougu Xiaotong capsule promotes the proliferation and differentiation of osteoblasts

doi:10.3969/j.issn.2095-4344.2013.33.004

Chinese Journal of Tissue Engineering Research

Tougu Xiaotong capsule promotes the proliferation and differentiation of osteoblasts

Huang Yun-mei1, 2, 3, Chen Wen-lie1, 2, 3, Lin Ru-hui1, 2, 3, Huang Mei-ya1, 2, 3, Li Zuan-fang1, 2, 3,
Liao Nai-shun1, 2, 3, Liu Xian-xiang1, 2, 3

1Fujian Academy of Integrative Medicine, 2Fujian Key Laboratory of Integrative Medicine on Geriatrics, 3National Laboratory of Traditional Chinese Medicine-Pharmacology (Cell Structure and Function), Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China

Received:2013-05-16 Revised:2013-07-13 Online:2013-08-13 Published:2013-08-13
Contact: Chen Wen-lie, Researcher, Master’s supervisor, 1Fujian Academy of Integrative Medicine, 2Fujian Key Laboratory of Integrative Medicine on Geriatrics, 3National Laboratory of Traditional Chinese Medicine-Pharmacology (Cell Structure and Function), Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China chen.wl@163.com Liu Xian-xiang, M.D., Professor, Doctoral supervisor, 1Fujian Academy of Integrative Medicine, 2Fujian Key Laboratory of Integrative Medicine on Geriatrics, 3National Laboratory of Traditional Chinese Medicine-Pharmacology (Cell Structure and Function), Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China liuxianxiang@163.com
About author:Huang Yun-mei★, Master, Assistant researcher, 1Fujian Academy of Integrative Medicine, 2Fujian Key Laboratory of Integrative Medicine on Geriatrics, 3National Laboratory of Traditional Chinese Medicine-Pharmacology (Cell Structure and Function), Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian Province, China huangym003@163.com
Supported by:
Fujian Natural Science Foundation, No. 2010J01200*; Chen Keji Development Foundation for Integrated Traditional and Western, No. CKJ2009005*, CKJ2011004*; Open Project of Fujian Key Laboratory, No. 2008J1004-29*

Abstract

Abstract:

BACKGROUND: Tougu Xiaotong capsule is the clinical prescription for the treatment of osteoarthritis in Fujian University of Traditional Chinese Medicine, and the previous studies mainly focus on effect to cartilage.
OBJECTIVE: To observe the effect of Tougu Xiaotong capsule on the proliferation and differentiation of osteoblasts as well as the expressions of bone remodeling correlated factors.
METHODS: Rat osteoblast-like cell line ROS17/2.8 cells were incubated with Tougu Xiaotong capsule. The ROS17/2.8 cells were divided into blank control group and Tougu Xiaotong capsule groups with different concentrations. The cell proliferation was determined by methylthiazolyldiphenyl-tetrazolium bromide assay. Osteoblast differentiation biomarkers alkaline phosphatase activity, osteocalcin and bone mineralized nodules were measured with colorimetry, enzyme-linked immunosorbent assay and alizarin red staining, respectively. The real-time PCR and enzyme-linked immunosorbent assay were used to detect the expressions of bone remodeling factors osteoprotegerin/receptor activator of nuclear factor κB ligand.
RESULTS AND CONCLUSION: Compared with the control group, the Tougu Xiaotong capsule with the concentration of 0.25-2 g/L could significantly promote the ROS17/2.8 cell proliferation (P < 0.05), up-regulate the alkaline phosphatase activity, osteocalcin expression level and mineralized nodules area, and increase the percentage of bone remodeling factors osteoprotegerin/receptor activator of nuclear factor κB ligand (P < 0.05). The mechanism of Tougu Xiaotong capsule protecting osteoarthritis may partly result from the regulation of proliferation and differentiation of osteoblasts and bone remodeling.

Key words: tissue construction, bone tissue construction, Tougu Xiaotong capsule, osteoarthritis, subchondral bone, osteoblasts, proliferation, osteoprotegerin/receptor activator of nuclear factor κB ligand, ,
bone remodeling, provincial grants-supported paper

CLC Number:

R318

Huang Yun-mei, Chen Wen-lie, Lin Ru-hui, Huang Mei-ya, Li Zuan-fang, . Tougu Xiaotong capsule promotes the proliferation and differentiation of osteoblasts[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.33.004.

Figures/Tables 6

References

[1] Hunter DJ. Osteoarthritis. Best Pract Res Clin Rheumatol. 2011;25(6):801-814.

[2] Ding M, Danielsen CC, Hvid I. Age-related three-dimensional microarchitectural adaptations of subchondral bone tissues in guinea pig primary osteoarthrosis. Calcif Tissue Int. 2006;78 (2):113-122.

[3] Goldring SR. Role of bone in osteoarthritis pathogenesis. Med Clin North Am. 2009;93(1):25-35, xv.

[4] Hayami T, Pickarski M, Zhuo Y, et al. Characterization of articular cartilage and subchondral bone changes in the rat anterior cruciate ligament transection and meniscectomized models of osteoarthritis. Bone. 2006;38(2):234-243.

[5] Kwan Tat S, Lajeunesse D, Pelletier JP, et al. Targeting subchondral bone for treating osteoarthritis: what is the evidence? Best Pract Res Clin Rheumatol. 2010;24(1):51-70.

[6] Castañeda S, Roman-Blas JA, Largo R, et al. Subchondral bone as a key target for osteoarthritis treatment. Biochem Pharmacol. 2012;83(3):315-323.

[7] Hunter DJ, Spector TD. The role of bone metabolism in osteoarthritis. Curr Rheumatol Rep. 2003;5(1):15-19.

[8] Bellido M, Lugo L, Roman-Blas JA, et al. Subchondral bone microstructural damage by increased remodelling aggravates experimental osteoarthritis preceded by osteoporosis. Arthritis Res Ther. 2010;12(4):R152.

[9] Carbone LD, Nevitt MC, Wildy K, et al. The relationship of antiresorptive drug use to structural findings and symptoms of knee osteoarthritis. Arthritis Rheum. 2004;50(11):3516-3525.

[10] Papaioannou NA, Triantafillopoulos IK, Khaldi L, et al. Effect of calcitonin in early and late stages of experimentally induced osteoarthritis. A histomorphometric study. Osteoarthritis Cartilage. 2007;15(4):386-395.

[11] Bellido M, Lugo L, Roman-Blas JA, et al. Improving subchondral bone integrity reduces progression of cartilage damage in experimental osteoarthritis preceded by osteoporosis. Osteoarthritis Cartilage. 2011;19(10): 1228-1236.

[12] Christgau S, Tankó LB, Cloos PA, et al. Suppression of elevated cartilage turnover in postmenopausal women and in ovariectomized rats by estrogen and a selective estrogen- receptor modulator (SERM). Menopause. 2004; 11(5):508-518.

[13] 李西海,陈文列,刘献祥.补肾柔肝法防治骨性关节炎作用机制探讨[J].福建中医药大学学报,2011,21(2):66-69.

[14] 林木南,刘献祥.透骨消痛方治疗膝骨性关节炎30例[J].福建中医药,2005,36(4):5-16

[15] 刘献祥,李西海,周江涛,等.透骨消痛胶囊防治膝骨性关节炎的机理研究[J].中国中西医结合杂志,2007,27(1):50-54.

[16] Zheng CS, Ye HZ, Xu XJ, et al. Computational pharmacology study of tougu xiaotong granule in preventing and treating knee osteoarthritis. Chin J Integr Med. 2009;15(5):371-376.

[17] 吴追乐,李西海,吴广文,等.透骨消痛胶囊含药血清对软骨细胞线粒体凋亡通路的影响[J].中华中医药杂志,2011,26(2):343-346.

[18] 叶蕻芝,李西海,梁文娜,等.透骨消痛胶囊含药血清对兔软骨细胞增殖影响的研究[J].中国临床药理学与治疗学,2009,14(6): 624-628.

[19] 黄云梅,陈文列,刘献祥,等.透骨消痛胶囊对骨性关节炎影响的组织化学研究[J].中国中医骨伤科学杂志,2011,19(1):1-3.

[20] 李民,郭逸尔,刘献祥,等.透骨消痛胶囊干预膝骨性关节炎软骨下骨重塑的分子机制[J].中国组织工程研究,2012,16(15): 2669- 2673.

[21] Nakayama Y, Yang L, Takai H, et al. Fibroblast growth factor 2 and forskolin induce mineralization-associated genes in two kinds of osteoblast-like cells. J Oral Sci. 2012;54(3):251-259.

[22] Chen H, Kolman K, Lanciloti N, et al. p53 and MDM2 are involved in the regulation of osteocalcin gene expression. Exp Cell Res. 2012;318(8):867-876.

[23] Alaiyan B, Ilyayev N, Stojadinovic A, et al. Differential expression of colon cancer associated transcript1 (CCAT1) along the colonic adenoma-carcinoma sequence. BMC Cancer. 2013;13(1):196.

[24] Trouvin AP, Goëb V. Receptor activator of nuclear factor-κB ligand and osteoprotegerin: maintaining the balance to prevent bone loss. Clin Interv Aging. 2010;5:345-354.

[25] Pivonka P, Zimak J, Smith DW, et al. Theoretical investigation of the role of the RANK-RANKL-OPG system in bone remodeling. J Theor Biol. 2010;262(2):306-316.

[26] Liu C, Walter TS, Huang P, et al. Structural and functional insights of RANKL-RANK interaction and signaling. J Immunol. 2010;184(12):6910-6919.

[27] Boyce BF, Xing L. Functions of RANKL/RANK/OPG in bone modeling and remodeling. Arch Biochem Biophys. 2008; 473(2):139-146.

[28] Sakao K, Takahashi KA, Mazda O, et al. Enhanced expression of interleukin-6, matrix metalloproteinase-13, and receptor activator of NF-kappaB ligand in cells derived from osteoarthritic subchondral bone. J Orthop Sci. 2008;13(3): 202-210.

Tougu Xiaotong capsule promotes the proliferation and differentiation of osteoblasts

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Peng Zhihao, Feng Zongquan, Zou Yonggen, Niu Guoqing, Wu Feng. Relationship of lower limb force line and the progression of lateral compartment arthritis after unicompartmental knee arthroplasty with mobile bearing [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(9): 1368-1374.
[2]	Huang Dengcheng, Wang Zhike, Cao Xuewei. Comparison of the short-term efficacy of extracorporeal shock wave therapy for middle-aged and elderly knee osteoarthritis: a meta-analysis [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(9): 1471-1476.
[3]	Liu Xiangxiang, Huang Yunmei, Chen Wenlie, Lin Ruhui, Lu Xiaodong, Li Zuanfang, Xu Yaye, Huang Meiya, Li Xihai. Ultrastructural changes of the white zone cells of the meniscus in a rat model of early osteoarthritis [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(8): 1237-1242.
[4]	Wang Zhengdong, Huang Na, Chen Jingxian, Zheng Zuobing, Hu Xinyu, Li Mei, Su Xiao, Su Xuesen, Yan Nan. Inhibitory effects of sodium butyrate on microglial activation and expression of inflammatory factors induced by fluorosis [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(7): 1075-1080.
[5]	Wang Shiqi, Zhang Jinsheng. Effects of Chinese medicine on proliferation, differentiation and aging of bone marrow mesenchymal stem cells regulating ischemia-hypoxia microenvironment [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(7): 1129-1134.
[6]	Li Cai, Zhao Ting, Tan Ge, Zheng Yulin, Zhang Ruonan, Wu Yan, Tang Junming. Platelet-derived growth factor-BB promotes proliferation, differentiation and migration of skeletal muscle myoblast [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(7): 1050-1055.
[7]	Liu Cong, Liu Su. Molecular mechanism of miR-17-5p regulation of hypoxia inducible factor-1α mediated adipocyte differentiation and angiogenesis [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(7): 1069-1074.
[8]	Liu Xin, Yan Feihua, Hong Kunhao. Delaying cartilage degeneration by regulating the expression of aquaporins in rats with knee osteoarthritis [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(5): 668-673.
[9]	Ma Zetao, Zeng Hui, Wang Deli, Weng Jian, Feng Song. MicroRNA-138-5p regulates chondrocyte proliferation and autophagy [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(5): 674-678.
[10]	Cao Xuhan, Bai Zixing, Sun Chengyi, Yang Yanjun, Sun Weidong. Mechanism of “Ruxiang-Moyao” herbal pair in the treatment of knee osteoarthritis based on network pharmacology [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(5): 746-753.
[11]	Li Yonghua, Feng Qiang, Tan Renting, Huang Shifu, Qiu Jinlong, Yin Heng. Molecular mechanism of Eucommia ulmoides active ingredients treating synovitis of knee osteoarthritis: an analysis based on network pharmacology [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(5): 765-771.
[12]	Song Shan, Hu Fangyuan, Qiao Jun, Wang Jia, Zhang Shengxiao, Li Xiaofeng. An insight into biomarkers of osteoarthritis synovium based on bioinformatics [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(5): 785-790.
[13]	Liu Bo, Chen Xianghe, Yang Kang, Yu Huilin, Lu Pengcheng. Mechanism of DNA methylation in exercise intervention for osteoporosis [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(5): 791-797.
[14]	Deng Zhenhan, Huang Yong, Xiao Lulu, Chen Yulin, Zhu Weimin, Lu Wei, Wang Daping. Role and application of bone morphogenetic proteins in articular cartilage regeneration [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(5): 798-806.
[15]	Wang Yujiao, Liu Dan, Sun Song, Sun Yong. Biphasic calcium phosphate loaded with advanced platelet rich fibrin can promote the activity of rabbit bone marrow mesenchymal stem cells [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(4): 504-509.