Bone morphogenetic protein 2 expression in rabbit radial defect site with different lengths

doi:10.3969/j.issn.2095-4344.2013.30.015

Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (30): 5511-5517.doi: 10.3969/j.issn.2095-4344.2013.30.015

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Bone morphogenetic protein 2 expression in rabbit radial defect site with different lengths

Zhan Yu-lin¹, An Zhi-quan¹, Sun Lu-yuan¹, Zhang Chang-qing¹, Zeng Bing-fang¹, Xu Feng², Hou Guo-zhu³, Li Wen-ju², Zhu Xiao-meng², Song Xing-hua²

1Department of Orthopedics, the Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200033, China
2Department of Orthopedics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
3Department of Orthopedics, the 152 Central Hospital of PLA, Pingdingshan 467000, Henan Province, China

Received:2013-04-09 Revised:2013-05-21 Online:2013-07-23 Published:2013-07-23
Contact: Zhan Yu-lin and Xu Feng contributed equally to this paper. Zhan Yu-lin, M.D., Associate chief physician, Department of Orthopedics, the Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200033, China bondwe@163.com Song Xing-hua, M.D., Associate professor, Chief physician, Master’s supervisor, Department of Orthopedics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China songxinhua19@yahoo.com.cn
About author:Zhan Yu-lin☆, M.D., Associate chief physician, Department of Orthopedics, the Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200033, China bondwe@163.com Xu Feng★, Master, Department of Orthopedics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China xufeng798@163.com
Supported by:
National Natural Science Foundation of China, No. 81060144

Abstract

Abstract:

BACKGROUND: It has been studied that the distribution of bone morphogenetic protein 2 is regular under bone defect situation.
OBJECTIVE: To observe the expression of bone morphogenetic protein 2 in rabbit radial defect site with different lengths.
METHODS: Forty-eight New Zealand rabbits were divided into two groups randomly, 0.5 cm bone defect and 3.0 cm bone defect were made by wire saw at the middle part of radius bone after anaesthesia.
RESULTS AND CONCLUSION: Western blot results showed that in the 0.5 cm bone defect group, the expression of bone morphogenetic protein 2 of the tissues in the bone defect site was increased gradually at 1, 3, 4 weeks after operation, and the expression in each defect group was increased when compared with that immediately after injury (P < 0.05). In the 3.0 cm bone defect group, the expression of bone morphogenetic protein 2 of tissues in bone defect site was increased gradually and reached to its peak at 3 weeks after the operation (P < 0.05), and the peak value in the 3.0 cm bone defect group was significantly higher than that in
0.5 cm bone defect group (P < 0.05). The peak value was maintained in high level. The comparison of bone callus formation showed that the bone callus formation of 3.0 cm bone defect group was less than that of the 0.5 cm bone defect group at 3 and 4 weeks after operation (P < 0.05). The results indicate that expression of the bone morphogenetic protein 2 in 3.0 cm bone defect site is increased significantly, but the expression level cannot make the bone defect heal itself.

Key words: bone and joint implants, basic experiment of bone joint injury, bone defect, bone morphogenetic protein 2, length, bone healing, rabbits, radius bone, National Natural Science Foundation of China

CLC Number:

R318

Zhan Yu-lin, An Zhi-quan, Sun Lu-yuan, Zhang Chang-qing, Zeng Bing-fang, Xu Feng, Hou Guo-zhu, Li Wen-ju, Zhu Xiao-meng, Song Xing-hua . Bone morphogenetic protein 2 expression in rabbit radial defect site with different lengths[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(30): 5511-5517.

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References

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Bone morphogenetic protein 2 expression in rabbit radial defect site with different lengths

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