Liuwei Dihuang Wan serum influences the expression of type Ⅰ and type Ⅱ
collagen in intervertebral discs

doi:10.3969/j.issn.2095-4344.2013.26.015

Abstract

Abstract:

BACKGROUND: Collagen fiber is the main subject to maintain the structural integrity and its physiological function, the type and content of the collagen in intervertebral disc will influence the physical and pathological condition of entire intervertebral disc. Type Ⅰ and type Ⅱcollagen are the main components of intervertebral disc collagen fiber.
OBJECTIVE: To observe the effect of Liuwei Dihuang Wan-containing serum on expression of type Ⅰ and type Ⅱ collagen and genes of the intervertebral discs in tumor necrosis factor-α injured rabbit.
METHODS: Forty-five L2-L5 intervertebral discs harvested from 15 rabbits were randomly divided into five groups: control 1st day, control 14th day, tumor necrosis factor-α group, Liuwei Dihuang Wan-containing serum group and tumor necrosis factor-α+ Liuwei Dihuang Wan-containing serum group. The culture medium in the Liuwei Dihuang Wan-containing serum group and tumor necrosis factor-α+ Liuwei Dihuang Wan-containing serum group contained
5 μg/L tumor necrosis factor-α and 10% Liuwei Dihuang Wan-containing serum. The nucleus pulposus and annulus fibrosus were collected after cultured for 14 days.
RESULTS AND CONCLUSION: Reverse transcription-PCR and Western Blot showed that with the culture time prolonging, the expressions of type Ⅰ collagen and genes in nucleus pulposus and type Ⅱ collage and genes in the annulus fibrosus were significantly decreased; Tumor necrosis factor-α could aggravate the change, while Liuwei Dihuang Wan-containing serum could delay the changes. The results indicate that Liuwei Dihuang Wan can protect the extracellular matrix of intervertebral discs through stabilizing the expressions of type Ⅰ and type Ⅱ collagen and genes.

Key words: bone and joint implants, basic experiment of spinal injury, Liuwei Dihuang Wan, discogenic low back pain, type Ⅰ collagen, type Ⅱ collagen, intervertebral disc, provincial grants-supported paper

CLC Number:

R318

Xu Wu-ji, Li Yue, Yuan Chao. Liuwei Dihuang Wan serum influences the expression of type Ⅰ and type Ⅱ
collagen in intervertebral discs[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.26.015.

Figures/Tables 4

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