A Wistar rat model of radiation-induced masseter injury

doi:10.3969/j.issn.2095-4344.2013.24.021

Abstract

Abstract:

BACKGROUND: Construction of a stable animal model of radiation-induced masseter injury is significant for the research of maxillofacial region tumor.
OBJECTIVE: To establish the animal model of Wistar rats with radiation-induced masseter injury after irradiated with the radiation dose of 40 Gy.
METHODS: A total of 20 male adult Wistar rats were selected and randomly divided into radiation injury group and control group. Radiation injury group was radiated with linear accelerator at a dose of 40 Gy in the masseter tissue. After irradiated for 28 days, pathological changes in the masseter tissue of the radiation injury group and control group were observed by light microscope and electron microscope; the gene expression of transforming growth factor-β1 was detected by reverse transcription-PCR.
RESULTS AND CONCLUSION: After irradiated for 28 days at the dose of 40 Gy, the irradiation injury of structural damage and reduced vascular density were observed in the masseter tissue (P < 0.01), and the gene expression of transforming growth factor-β1 was increased (P < 0.001). The result indicates that masseter tissue irradiated with linear accelerator at a dose of 40 Gy can be considered as the model of radiation-induced masseter injury.

Key words: tissue construction, experimental modeling in tissue construction, radiation injury, animal model, transforming growth factor-beta 1, vascular density, masseter, radiation therapy, maxillofacial, ultrastructure, gene expression, pathological changes, other grants-supported paper

CLC Number:

R318

Dong Gang, Zheng Jian-jin, Li Tao, Xu Xin, Lu Shu-lai. A Wistar rat model of radiation-induced masseter injury[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(24): 4515-4520.

Figures/Tables 4

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