Expression and significance of stromal cell derived factor-1 in the intervertebral disk after lumbar disc degeneration

doi:10.3969/j.issn.2095-4344.2013.24.017

Abstract

Abstract:

BACKGROUND: Exact pathogenesis of lumbar disc degeneration is not very clear. Inflammation is involved in the mechanism of lumbar disc degeneration. Stromal cell derived factor-1, which belongs to chemokine family, is related to inflammation.
OBJECTIVE: To determine the expression of stromal cell derived factor-1 in the intervertebral disk of patients with lumbar disc degeneration, and then to analyze the association of the expression levels of stromal cell derived factor-1 with the severity of lumbar disc degeneration.
METHODS: Eighty-four patients with lumbar disc degeneration (lumbar disc degeneration group) and 28 patients with disc burst fractures (control group) were enrolled in this study. The intervertebral disk tissues were collected after surgery. The protein level of stromal cell derived factor-1 was determined by enzyme linked immunosorbent assay. The grade of disc degeneration was determined according to Schneiderman’s classification. Association between the levels of stromal cell derived factor-1 and the Schneiderman’s grade was analyzed.
RESULTS AND CONCLUSION: The stromal cell derived factor-1 levels in the intervertebral disk were significantly elevated in the lumbar disc degeneration group compared with the control group (P < 0.01). The levels of stromal cell derived factor-1 were significantly increased in lumbar disc degeneration patients with grade 4 compared with those with grades 2 and 3. Furthermore, lumbar disc degeneration patients with grade 3 showed significantly elevated stromal cell derived factor-1 levels compared with those with grade 2. Spearman correlation analysis indicated that stromal cell derived factor-1 levels were positively correlated with Schneiderman’s grades (r=0.412, P < 0.01). These findings indicate that the levels of stromal cell derived factor-1 are significantly elevated in the intervertebral disk of patients with lumbar disc degeneration, and positively associated with the severity of lumbar disc degeneration. Stromal cell derived factor-1 may be involved in the mechanism of lumbar disc degeneration.

Key words: tissue construction, tissue construction and bioactive factors, lumbar disc degeneration, stromal cell derived factor-1, expression, inflammation, chemokines, Schneiderman’s grades, cytokines, enzyme-linked immunosorbnent assay, extracellular matrix, matrix metalloproteinase

CLC Number:

R318

Liu Gang, Ma Xin-long, Deng Shu-cai, Chen Si. Expression and significance of stromal cell derived factor-1 in the intervertebral disk after lumbar disc degeneration[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(24): 4488-4494.

Figures/Tables 2

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