Nucleus pulposus cell transplantation inhibits intervertebral disk degeneration

doi:10.3969/j.issn.2095-4344.2013.53.023

Abstract

Abstract:

BACKGROUND: In recent years, cell transplantation and intervertebral disk grafting for intervertebral disk degeneration have been greatly developed.

OBJECTIVE: To investigate the in vitro culture methods of nucleus pulposus cells and effects of nucleus pulposus cell transplantation on intervertebral disk degeneration.

METHODS: A computer-based search of databases was performed to explore the effects of nucleus pulposus cell transplantation on intervertebral disk degeneration. The main function of the nucleus pulposus cells is to produce collagen and proteoglycans. Through improvement of culture methods, the number of nucleus pulposus cells increased, and matrix synthesis and secretion also increased. Therefore, the nucleus pulposus cell transplantation was able to repair the degenerated intervertebral disk.

RESULTS AND CONCLUSION: The three-dimensional aggregation culture method and three-dimensional microcarrier rotation method elicit the increase in the number of nucleus pulposus cells, and hepatocyte growth factor also can produce a catalytic role in the proliferation of nucleus pulposus cells. Nucleus pulposus cell transplantation can restore degenerative disc height, and promote the biosynthesis and secretion of proteoglycans and type Ⅱ collagen in the degenerated intervertebral disk. With the deepening of intervertebral disk research, nucleus pulposus cell transplantation may become an important therapeutic tool for intervertebral disk degeneration.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: cell transplantation, intervertebral disk, intervertebral disk degeneration, extracellular matrix, collagen type Ⅱ, proteoglycans

CLC Number:

R318

Wang Chun-sheng, Zhang Wei-bin. Nucleus pulposus cell transplantation inhibits intervertebral disk degeneration[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(53): 9239-9244.

Figures/Tables 8

在不同时间点各组磁共振ST2WI数据显示，第8周时髓核细胞移植组T2信号强度(70.63±7.60)%高于骨髓基质细胞移植组(54.32±7.92)%，两组比较差异有显著性意义(P < 0.05)。提示髓核细胞移植组细胞外基质的合成可能超过骨髓基质细胞移植组。可见，髓核细胞移植或骨髓基质细胞移植能够有效避免椎间盘损伤后发生的进行性退变过程。而且，在8周后髓核细胞移植组较骨髓基质细胞移植组显示出更强的基质合成能力。椎间盘高度及含水量出现恢复的迹象。外源性髓核细胞移植能够良好的适应受体椎间盘环境，并发挥相应的细胞外基质分泌功能。 2.2.2 髓核细胞移植促进退变椎间盘内蛋白多糖和Ⅱ型胶原的生物合成刘勇[19]在微创经皮CT引导下穿刺椎间盘建立兔椎间盘退变模型，将60只新西兰大白兔分别用甲苯噻嗪注射液3 mg/kg、克他命注射液40 mg/kg肌肉注射麻醉后，兔取左侧卧位，腰背部正中及左侧穿刺区域备皮，以2.5%的碘伏消毒，选择椎间盘层面行CT扫描以穿刺造模，行腰椎间盘CT扫描，以定位穿刺椎间隙。兔左后方腰背部为进针点，消毒后在CT定位下，经皮以18G的穿刺针由左后方穿刺腰间盘。穿刺针尖接近纤维环时行CT扫描，调整穿刺角度及深度，缓缓进针，刺入髓核后再次CT扫描，以确定针尖位于髓核中央。对兔椎间盘细胞进行培养，在体外转双基因，将腺相关病毒介导的结缔组织生长因子和基质金属蛋白酶组织抑制因子1双基因体外联合转染兔椎间盘髓核细胞后，再将转基因髓核细胞移植于退变兔腰椎间盘内，通过免疫组织化学和反转录聚合酶链反应以及35S整合法检测髓核细胞移植对椎间盘退变的影响，观察不同时期椎间盘信号、椎间高度、Ⅱ型胶原和蛋白多糖合成的变化。发现在体外转双基因腺相关病毒介导的结缔组织生长因子和基质金属蛋白酶组织抑制因子1髓核细胞移植可以延缓或逆转椎间盘的退变。研究中的几项检测结果发现，见表6。"

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