c-fos/c-jun regulates extracellular matrix metalloproteinase 20 expression in ameloblasts

doi:10.3969/j.issn.2095-4344.2015.11.007

Abstract

Abstract:

BACKGROUND: Matrix metalloproteinase 20 is a protease specifically expressed in ameloblasts, which has an important role in dental enamel development. To study the regulatory mechanisms for matrix metalloproteinase 20 at the molecular level lays the foundation for further animal experiments.

OBJECTIVE: To explore the regulatory effects of transcription factor c-fos/c-jun for matrix metalloproteinase 20 in mouse ameloblasts and to preliminarily confirm the role of c-fos/c-jun in enamel development.

METHODS: First of all, a recombinant plasmid containing c-fos was established, and then dual-luciferase reporter assay system and RT-PCR were used to analyze the effects of c-fos, c-jun transfection of ameloblasts on the activity of matrix metalloproteinase 20. Furthermore, the effect of c-fos, c-jun on matrix metalloproteinase 20 was explored based on gene site-directed mutation and dual-luciferase reporter assay system.

RESULTS AND CONCLUSION: Double luciferase report assay system and RT-PCR analysis showed that the mRNA expression of matrix metalloproteinase-20 was significantly upregulated after c-fos, c-jun transfection of ameloblasts, but c-fos/c-jun could not upregulate the transcriptional activity of matrix metalloproteinase 20 promoter when mutation occurred at AP1 binging site. These findings indicate that c-fos/c-jun has significant effects in regulating the mRNA expression of matrix metalloproteinase 20, which shows c-fos/c-jun plays an important biological meaning in enamel development.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: Proto-Oncogene Proteins c-fos, Matrix Metalloproteinase 20, Dental Enamel

CLC Number:

R318

Tang Pei-juan, Wang Chang-lei, Gong Chun-mei, Tang Pei-qian, Hao Jian-zhong. c-fos/c-jun regulates extracellular matrix metalloproteinase 20 expression in ameloblasts [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(11): 1673-1677.

Figures/Tables 2

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