Pigment epithelium-derived factor inhibits corneal neovascularization after corneal alkali burn

doi:10.3969/j.issn.2095-4344.2013.20.014

Abstract

Abstract:

BACKGROUND: Corneal neovascularization can reduce the transparency of cornea thus causing severe visual impairment. Pigment epithelium-derived factor is an endogenous angiogenesis inhibitor, and it is unclear whether it can inhibit corneal neovascularization.
OBJECTIVE: To investigate the inhibitory effect of topically administration of pigment epithelium-derived factor on the corneal neovascularization of rats after corneal alkali burn.
METHODS: Twenty rats were randomly divided into normal saline group and pigment epithelium-derived factor group, 10 rats in each group. NaOH solution was used to burn the right cornea in order to induce neovascularization. After burn, the rats in the two groups received pigment epithelium-derived factor drop and normal saline drop daily, respectively, and the growth of corneal neovascularization in each group was observed and calculated by slit lamp microscope. The rats were sacrificed at 12 days after burn, and then the histological structure of the cornea was observed with hematoxylin-eosin staining. Expression of vascular endothelial growth factor and CD31 was detected by immunohistochemistry．
RESULTS AND CONCLUTION: At 3, 7 and 12 days after corneal alkali burn, the area of corneal neovascularization in the pigment epithelium-derived factor group was smaller than that in the normal saline group (P < 0.05). At 12 days after corneal alkali burn, hematoxylin-eosin staining revealed that a large amount of neovascularization could be seen in the normal saline group, and the structure of corneal tissue was in disorder; the neovascularization was less in the pigment epithelium-derived factor group, and the structure of corneal tissue was in order. Immunohistochemical staining at 12 days after corneal alkali burn revealed that the expression of vascular endothelial growth factor was mainly in corneal epithelial layer and matrix layer. The expression of vascular endothelial growth factor and CD31 was massive in corneal matrix layer; while the corneal neovascularization was less in the pigment epithelium-derived factor group and the CD31 expression was weak. The experimental results indicate that topical application of pigment epithelium-derived factors can inhibit alkali-induced corneal neovascularization effectively.

Key words: tissue consteuction, tissue construction and bioactive factors, cornea, corneal neovascularization, pigment epithelium-derived factor, NaOH, vascular endothelial growth factor, CD31, ophthalmic tissue engineering

CLC Number:

R318

Zhu Dan, Lu Xiao-he, Fu Yue. Pigment epithelium-derived factor inhibits corneal neovascularization after corneal alkali burn [J]. Chinese Journal of Tissue Engineering Research, 2013, 17(20): 3707-3714.

Figures/Tables 4

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