Transforming growth factor beta 1 and beta 3 expression during skin wound healing of rats

doi:10.3969/j.issn.2095-4344.2013.20.005

Abstract

Abstract:

BACKGROUND: Transforming growth factor β plays a key role in the repair of tissue injury.
OBJECTIVE: To investigate the expression level and distribution of transforming growth factor β1 and transforming growth factor β3 during healing of skin scarred wound.
METHODS: The full-thickness incised wound model was established in rats, 1.5-2.0 cm long to the fascia layer. Immunohistochemistry was used to quantitatively analyze the expression level and position of transforming growth factor β1 and transforming growth factor β3 in rats at 0, and 12 hours, 1, 2, 3, 4, 5, 6, and 7 days post injury.
RESULTS AND CONCLUSION: Results showed that the transforming growth factor β1 and transforming growth factor β3 positive particles mainly distributed in cytoplasm of epithelial cells and epithelial basilar membrane, macrophage and granulation tissue in the early stage of wound healing (1-5 days post injury). With prolonged time, the positive particles were mainly found in fibroblast and extracellular matrix. The expression level of transforming growth factor β1 was up-regulated intensively during 1-5 days after injury, and transforming growth factor β3 was significantly expressed since 6 and 7 days post injury. That is, transforming growth factor β1 was earlier expressed than transforming growth factor β3 during wound healing. Results showed a close relationship between transforming growth factor β1 and collagen formation, as well as wound repair. The expression level of transforming growth factor β3 was increased in the later stage of wound healing, indicating that transforming growth factor β3 may be related to the remodeling of the wound.

Key words: tissue construction, skin tissue construction, scar, wound healing, skin, transforming growth factor beta 1, transforming growth factor beta 3, granulation tissue, fibroblast, macrophage, cytokine, National Natural Science Foundation of China

CLC Number:

R318

Zhao Jia-jia, Yang Pi-bo, Han Chuan-huo, Liu Jia-rong. Transforming growth factor beta 1 and beta 3 expression during skin wound healing of rats[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(20): 3643-3649.

Figures/Tables 3

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