Expression of chemokine signaling pathway in the subchondral bone of early experimental osteoarthritis

doi:10.3969/j.issn.2095-4344.2013.11.004

Abstract

Abstract:

BACKGROUND: Chemotatic factors distribute widely in the inflammatory tissues, which can induce inflammatory cells to participate in immune response. Large experimental results have shown that chemotatic factors exert an important role in pathogenesis of osteoarthritis.
OBJECTIVE: To study the expression of chemokine signaling pathway in the subchondral bone of early experimental osteoarthritis, which provides evidences that this signaling pathway plays an important role in the pathogenesis of osteoarthritis.
METHODS: Thirty Sprague-Dawley rats were equally divided into two groups: experimental group and control group. The right knee joints of rats in the experimental group underwent both medial collateral ligament transection and medial meniscectomy, while a sham operation was carried out in the control group. Rats were killed at 1, 2 and 4 weeks postsurgery to obtain the right knee joints. Total RNA of the subchondral bone was extracted, and then hybridized to Agilent Whole Rat Genome Microarray. Differentially expressed genes analysis and pathway analysis were used to study the chemokine signaling pathway.
RESULTS AND CONCLUSION: (1) A set of differential expression genes known involved in the chemokine signaling pathway were found. (2) Chemokine signaling pathway was found expressed significantly in 1 week post-surgery in the experimental group as compared with the control group (P < 0.05), while it showed no changes at 2 and 4 weeks postsurgery (P > 0.05). Chemokine signaling pathway plays an important role in pathogenesis of very early experimental osteoarthritis.

Key words: tissue construction, cartilage tissue construction, gene chips, osteoarthritis, subchondral bone, chemokines, signaling pathway, meniscus, medial collateral ligament, inflammatory tissue, immune response, osteoblasts, the National 863 Program of China

CLC Number:

R318

Zhang Rong-kai, Ye Zhi-qiang, Chen Yan, Fang Hang, Zhang Song-yang, Wu Zhao-ya,Zhao Qing, Cai Dao-zhang. Expression of chemokine signaling pathway in the subchondral bone of early experimental osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(11): 1925-1930.

Figures/Tables 2

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