Chemokin CXC motif receptor 4 expression in bone tissue surrounding anode-oxidized implants during early implantation

doi:10.3969/j.issn.2095-4344.2013.09.011

Abstract

Abstract:

BACKGROUND: The anode-oxidized implants have shown high implant stability at early period compared to traditional implants. However, the cellular and molecular mechanisms are unknown.
OBJECTIVE: To investigate the significance of chemokin CXC motif receptor 4 in bone tissue around anode-oxidized implant during early implantation.
METHODS: Forty Wistar rats of 4 months old were selected and randomly divided into 6, 12 and 24 hours implantation groups and blank control group. Rats in experimental groups received anode-oxidized implant in one tibia and sand blast implant in the other one. Rats in the experimental groups were killed at 6, 12 and 24 hours accordingly after implantation, the implants were unscrewed, and the bone tissue around implants was prepared, 2 cm in diameter. For blank control group, proximal epiphysis bone tissue about 2 cm in diameter were prepared. Half bone tissues in the experimental groups were made into protein samples. The expression of chemokin CXC motif receptor 4 was detected by Western blot with β-actin as internal reference. The other half bone tissues were treated with neutral ethylene diamine tetraacetie acid, and then slides were made. The expression of chemokin CXC motif receptor 4 was detected by immunohistochemistry. The implants in the 24 hours implantation group were examined by scanning electron microscope.
RESULTS AND CONCLUSION: The results of Western blot demonstrated that the expression of chemokin CXC motif receptor 4 in rat bone tissue of the experimental groups were significantly higher than that of the blank control group within 24 hours after implantation. At the same time point, the expression of chemokin CXC motif receptor 4 in rat bone tissue around the anode-oxidized implant was higher than that around the sand blast implant. The results of immunohistochemistry were consistent with those of Western blot. The observation results of scanning electron microscope showed that anode-oxidized surface showed more attached mesenchymal-like cells scattered all over the surfaces than the sand blast implant. The anode-oxidized implants can induce higher expression of chemokin CXC motif receptor 4 around bone tissue, and may promote the stability of implant at early time points.

Key words: bone and joint implants, orthopedic implants, Western blot, scanning electron microscopy, chemokin CXC motif receptor 4, implants, immunohistochemistry, photographs-containing paper of bone and joint implants

CLC Number:

R318

Jia Ting-ting, Zhang Yun-tao, Liu Shun-zhen, Sun Xin, Hou Yu-dong. Chemokin CXC motif receptor 4 expression in bone tissue surrounding anode-oxidized implants during early implantation[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(9): 1592-1597.

Figures/Tables 6

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