Proliferation and migration of cardiomyocytes and cardiac fibroblasts cultured with stromal cell-derived factor-1 alpha

doi:10.3969/j.issn.2095-4344.2013.07.015

Abstract

Abstract:

BACKGROUND: New wound in the border of defected hearts can promote self-healing, and stromal cell-derived factor-1 alpha can promote angiogenesis and cardiac function.
OBJECTIVE: To investigate the effects of mechanical injury and stromal cell-derived factor-1 alpha on the proliferation of cardiomyocytes and the effects of cardiomyocytes on chemotactic migration of cardiac fibroblasts.
METHODS: Rat cardiomyocytes and cardiac fibroblasts were cultured primarily. Rat cardiomyocytes were injured mechanically by scratching, and treated with 10-160 μg/L stromal cell-derived factor-1 alpha. The Cell Counting Kit-8 assay was employed to evaluate the proliferation of cardiomyocytes and the Transwell migration assay was used to detect the chemotaxis and migration capability of cardiac fibroblasts.
RESULTS AND CONCLUSION: Under mechanical injury condition, stromal cell-derived factor-1 alpha significantly promoted the growth of cardiomyocytes with the maximum effect occurring at 80 μg/L. In addition, mechanically injured and stromal cell-derived factor-1 alpha cultured cardiomyocytes remarkably promoted the migration of cardiac fibroblasts in a time-dependent fashion. These findings indicate that stromal cell-derived factor-1 alpha could promote the growth of cardiomyocytes under mechanical injury condition; otherwise, mechanically injured and stromal cell-derived factor-1 alpha cultured cardiomyocytes could promote the migration of cardiac fibroblasts.

Key words: tissue construction, tissue construction and bioactive factors, cardiomyocytes, cardiac fibroblasts, stromal cell-derived factor 1 alpha, physical damage, congenital heart defects, atrial septal defects, ventricular septal defects, patent ductus arteriosus, proliferation, chemokines, the National Natural Science Foundation of China, tissue construction photographs-containing paper

CLC Number:

R318

Hou Chuan-ju, Qi Yan-mei, Zhang Duan-zhen, Wang Qi-guang, Cui Chun-sheng, Kuang Li, Chen Bing. Proliferation and migration of cardiomyocytes and cardiac fibroblasts cultured with stromal cell-derived factor-1 alpha[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(7): 1220-1227.

Figures/Tables 5

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