Establishment and evaluation of a rat model of chronic cervical spinal cord compression

doi:10.3969/j.issn.2095-4344.2013.07.011

Abstract

Abstract:

BACKGROUND: Establishing a stable model of cervical spondylotic myelopathy is essential for the study of cervical spondylotic myelopathy pathogenesis.
OBJECTIVE: To clarify the mechanism of cervical spondylotic myelopathy by establishing a cervical spondylotic myelopathy model and observing the pathophysical changes of this model.
METHODS: A total of 30 Sprague-Dawley rats were divided into three groups: control group, mild compression group and severe compression group. Water-absorbing compression materials of different size were implanted below the C5-7 to prepare chronic cervical spinal cord compression models. Control group was implanted nothing.
RESULTS AND CONCLUSION: The normal morphology in the control group and varying degrees of compression on the spinal cord and spinal canal stenosis in the two compression groups were shown in MRI. The lower amplitude and longer latency of motor evoked potential after spinal cord compression was found in the two compression groups as compared with the control group (P < 0.05). Immunofluorescence staining showed that that a great number of healthy-shaped neurons were found in the control group. But, the number of neurons in the two compression groups was significantly decreased, and the neurons shrunk and demyelination was obvious. The difference of neuron number among the three groups was statistically significant (P < 0.05). Apoptosis in the segment of compressed spinal cord was found in the two compression groups rather than in the control group. In conclusion, rat models of chronic spinal cord compression are in line with the pathological changes of cervical spondylotic myelopathy. The surgical procedure is simple and the rate of infection and mortality is lower. Neuronal injury, demyelination and apoptosis are involved in the process of chronic spinal cord compression in rats.

Key words: tissue construction, experimental modeling in tissue construction, chronic spinal cord compression, animal models, rats, histology, motor evoked potentials, demyelination, cervical spondylotic myelopathy, tissue construction photographs-containing paper, the National Natural Science Foundation of China

CLC Number:

R318

Wang Jun, Wei Feng, Wang Hui-liang, Zhao Jing, Rong Wei, Hu Xing, Liu Zhong-jun. Establishment and evaluation of a rat model of chronic cervical spinal cord compression[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(7): 1196-1200.

Figures/Tables 4

References

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