Abstract:

BACKGROUND: The diagnosis of deep vein thrombosis is mainly dependent on imaging and laboratory tests, and the most fatal lack is that thrombosis can be confirmed until it has been formed. Accordingly, it is necessary to explore inherent rules using molecular technology.
OBJECTIVE: To observe the Hmox-1 expression in the process of formation of traumatic deep vein thrombosis in rats and to discuss the feasibility of Hmox-1 as a molecular marker for predictive diagnosis of deep vein thrombosis.
METHODS: Bilateral femoral veins were clamped and both hind limbs were fixed in rats to prepare traumatic deep venous thrombosis models. According the modeling time and formation of thrombosis, 100 experimental rats were randomized into five groups: normal group, trauma group, pre-thrombosis group, group of thrombosis formation at peak stage, group of non-thrombosis formation at peak stage. Real time-PCR was used to detect blood Hmox-1 mRNA expression. Bilateral femoral veins were isolated for histological observation of thrombosis degree.
RESULTS AND CONCLUSION: At 25 hours after modeling, the thrombosis rate was 58.46% (38∕65). mRNA expression of Hmox-1 in the pre-thrombosis group and group of thrombosis formation at peak stage was higher than that of the normal group (P < 0.05). There was no significant difference in the Hmox-1 mRNA expression between the normal group and the group of non-thrombosis formation at peak stage. These findings indicate that the variation tendency of Hmox-1 in the blood is consistent with the biological process of thrombosis formation, and Hmox-1 may become a marker for predictive diagnosis of deep venous thrombosis.

Key words: tissue construction, cytological experiments of tissue construction, deep vein thrombosis, Hmox-1, predictive diagnosis, rats, thrombosis, animal models, tissue construction photographs- containing paper