Monosodium urate monohydrate crystals affect the tight junctions among renal tubular epithelial cells

doi:10.3969/j.issn.2095-4344.2013.02.018

Abstract

Abstract:

BACKGROUND: Up to now, the relationship of uric acid stones with tight junction proteins and renal interstitial fibrosis is yet unclear.
OBJECTIVE: To observe the effect of monosodium urate (MSU) monohydrate crystals on the tight junction among NRK-52E cells.
METHODS: The NRK-52E cells were divided into two groups: control group and MSU group cultured in serum-free medium and MSU crystals, respectively. The protein and mRNA expressions of ZO-1 were investigated by immunofluorescence and reverse transcription-PCR, respectively.
RESULTS AND CONCLUSION: Compared with the control group, the protein and mRNA expressions of ZO-1 were down-regulated after the stimulation of MSU at 24, 48, 72 hours (P < 0.05), especially at 72 hours (P < 0.01). Protein expression appeared with redistribution phenomenon. These findings indicate that MSU can damage the structure, function and distribution of tight junction proteins of renal tubular epithelial cells

Key words: tissue construction, cytological experiments of tissue construction, monosodium urate monohydrate crystals, tight junctions, claudin, tubular epithelial cells, NRK-52E cells, renal interstitial fibrosis, hyperuricemia, transmembrane protein, membranous protein, rats, tissue construction photographs-containing paper

CLC Number:

R318

Li Hui-juan, Chen Gan, Fu Jun-zhou, Zhou Shan-shan. Monosodium urate monohydrate crystals affect the tight junctions among renal tubular epithelial cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(2): 286-290.

Figures/Tables 2

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