Abstract:

BACKGROUND: To establish a model of osteoarthritis with traditional Chinese medicine syndromes is conductive to the syndrome differentiation studies.
OBJECTIVE: To establish a rat model of knee osteoarthritis with kidney deficiency and blood stagnation.
METHODS: A total of 30 female Sprague-Dawley rats aged 2 months, SPF grade, were randomly divided into normal group, knee osteoarthritis group and knee osteoarthritis with kidney deficiency and bloods stagnation group (combined group), 10 rats in each group. Knee osteoarthritis models were established using Hulth method, kidney deficiency models were established using ovariectomized method, and blood stagnation models were established by injection of adrenal corticosteroids and epinephrine. Combined models were prepared based on the above-mentioned three models. Kidney deficiency models were prepared first followed by establishment of knee osteoarthritis and bloods stagnation models.
RESULTS AND CONCLUSION: Rats in the combined group presented listlessness, non-flexible activities and loose stools at 3 weeks after establishment of kidney deficiency models, and coat lackluster, ptosis, obvious weight loss and blue tail at 6 weeks after establishment of bloods stagnation models. Rats in the combined group also showed that the reducing viscosity was significantly up-regulated and the platelet aggregation reinforced, with blood flow slow-moving, circulatory disorder and so on. Meanwhile, the articular cartilage of the knee osteoarthritis group showed cataplasia, but it was slighter compared with the combined group. The rat model of knee osteoarthritis with kidney deficiency and blood stagnation is established successfully, and kidney deficiency and blood stagnation can aggravate articular cartilage degeneration.

Key words: tissue construction, experimental modeling of tissue construction, kidney deficiency, blood stagnation, knee osteoarthritis, models, rats, provincial grants-supported paper, tissue construction photographs- containing paper