Ultrapulse CO2 fractional laser intervens in situ superficial scar regeneration in a rabbit ear

doi:10.3969/j.issn.2095-4344.2013.02.008

Abstract

Abstract:

BACKGROUND: The ultrapulse CO2 fractional laser has a good effect on the treatment of superficial scars, but the mechanism is unclear.
OBJECTIVE: To investigate the effect of ultrapulse CO2 fractional laser on the treatment of superficial scars in rabbit ear and to explore mechanism of wound in situ regeneration and repair.
METHODS: The superficial scars in rabbit ear were induced with ultrapulse CO2 fractional laser, then traditional iodophor treatment and moist exposed burn ointment treatment methods were used for repairing the wound.
RESULTS AND CONCLUSION: The wound healing time of moist exposed burn ointment group was shorter than that of iodophor group (P < 0.01). The epidermal stem cells emerged after the superficial scars induced with ultrapulse CO2 fractional laser, and the expression of cytokeratin 19 and P63 protein was observed after induced for 24 hours. The number of cytokeratin 19 and P63 protein in the moist exposed burn ointment was increased after induced for 4 days and reached peak at 7 days and then decreased at 14 days. While the number of cytokeratin 19 and P63 protein in the iodophor group was increased after induced for 4 days, and then decreased after induced for 7 and 14 days. The expression of cytokeratin 19 and P63 protein in the moist exposed burn ointment group was significantly higher than that in the iodophor group (P < 0.05). Ultrapulse CO2 fractional laser combined with moist exposed burn ointment for the treatment of superficial scar in rabbit ear can better activate and protect the epidermal stem cells, and promote wound repair and in situ regeneration.

Key words: tissue construction, skin tissue construction, ultrapulse CO2 laser, fractional laser, superficial scar, epidermal stem cells, cytokeratin 19, P63 protein, provincial grants-supported paper, tissue construction photographs-containing paper

CLC Number:

R318

Tan Jun, Lei Ying, Li Gao-feng, Ouyang Hua-wei, Zhu Yi, Li Bo. Ultrapulse CO₂ fractional laser intervens in situ superficial scar regeneration in a rabbit ear[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(2): 228-234.

Figures/Tables 7

References

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Ultrapulse CO₂ fractional laser intervens in situ superficial scar regeneration in a rabbit ear

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