Chinese Journal of Tissue Engineering Research ›› 2013, Vol. 17 ›› Issue (2): 205-209.doi: 10.3969/j.issn.2095-4344.2013.02.004

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Intraosseous injection of pure alcohol induces necrosis of the femoral head in a rabbit

Chen Dong1, Hua Wen-bin1, Ye Shu-nan1, Yang Shu-hua1, Chen Chao1, Wang Xiao-hong1, Yu Qian1, Liu Xian-zhe1, Suo Jin-ping2, Nie Lei2   

  1. 1 Department of Orthopedics, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China 2 Institute of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, Hubei Province, China
  • Received:2012-03-16 Revised:2012-05-17 Online:2013-01-08 Published:2013-01-08
  • Contact: Corresponding author: Yang Shu-hua, Chief physician, Doctoral supervisor, Professor, Department of Orthopedics, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China shuhuayabc@vip.sina.com Corresponding author: Ye Shu-nan, Doctor, Attending physician, Department of Orthopedics, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China shunanye@gmail.com
  • About author:Chen Dong☆, Studying for doctorate, Department of Orthopedics, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China cd2408@163.com
  • Supported by:

    Supported by: the Fundamental Research Funds for the Central Universities, No. 2010JC029*, 2011JC042*; the National Natural Science Foundation of China, No. 81171750*, 30973044

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Abstract:

BACKGROUND: Currently, it is difficult to prepare an animal model of early osteonecrosis of the femoral head (ONFH).
OBJECTIVE: To develop a simple and dependable animal model of ONFH that allows the evaluation of different therapeutic approaches.
METHODS: Pure alcohol was injected into the centre of the right femoral head in New Zealand white rabbits under X-ray fluoroscopy, and the left femoral head with no treatment served as controls. After 2, 4 and 6 weeks, the animals were killed under anesthesia and the femoral heads were harvested.
RESULTS AND CONCLUSION: Gross and X-ray observations indicated that the articular cartilage in the modeling side of the femoral head was dark in color and had the uneven bone density at the first two weeks after modeling. At 6 weeks, a slight depression was found on the articular surface, and the bone density shadow increased. MRI results showed that line or irregular T1-weighted signals and high T2-weighted signals were seen in the weight-bearing area of the femoral head at 2 weeks; femoral head degeneration, subchondral fracture, articular surface collapse, and crescent formation occurred at 6 weeks after modeling. In addition, there was no abnormality in the control side of the femoral head at 2, 4 and 6 weeks. Histopathological results showed karyopyknosis, degeneration and necrosis of the femoral head at 2 weeks after modeling. These findings verified that partial osteonecrosis was confirmed over a period of 2 weeks in all animals. The morphology of necrosis in combination with intact macrotexture and joint cartilage was similar to the features described in early ONFH in humans. We obtained a good efficacy and similarity animal model of the early stages of ONFH in humans. This model may be compatible to develop new therapeutic methods for ONFH.

Key words: tissue construction, bone tissue construction, femoral head necrosis, animal models, ethanol, rabbits, X-ray, MRI, National Natural Science Foundation of China, tissue construction photographs-containing paper

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