Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (48): 9050-9053.doi: 10.3969/j.issn.2095-4344.2012.48.023

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Expression of osteoprotegerin and vascular endothelial growth factor after local injection of connective tissue growth factor

Ding Ming-cong1, Wei Pan-deng2, Lin Qing2, Du Bin2, Tai Hui-ping3, Chen Zhi-xin3   

  1. 1Department of Emergency Medicine, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China
    2First Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu Province, China
    3First Department of Orthopedics, Gansu Provincial People's Hospital, Lanzhou 730000, Gansu Province, China
  • Received:2012-01-01 Revised:2012-05-17 Online:2012-11-25 Published:2013-03-14
  • Contact: Chen Zhi-xin, Chief physician, Master’s supervisor, First Department of Orthopedics, Gansu Provincial People's Hospital, Lanzhou 730000, Gansu Province, China zhixinchen2008@126.com
  • About author:Ding Ming-cong★, Master, Department of Emergency Medicine, Second Hospital of Lanzhou University, Lanzhou 730030, Gansu Province, China sdwfdmc@163.com

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Abstract:

BACKGROUND: Studies have found that connective tissue growth factor plays an important role in the cartilage development process with a strong angiogenic proliferative effect, but its influence on fracture healing is not clear.
OBJECTIVE: To explore the effects of connective tissue growth factor on the expression of osteoprotegerin and vascular endothelial growth factor during fracture healing and its mechanism.
METHODS: Forty Sprague Dawley rats were randomly divided into two groups. After the tibial bone fracture model was established, the rats in the experimental group were injected with 0.1 μg/kg connective tissue growth factor at the sites of the bone fracture, rats in the control group were injected with the same volume of normal saline. This was carried once every 2 days.
RESULTS AND CONCLUSION: The hematoxylin-eosin staining indicated that more chondrocytes were found at early-stage of callus in experimental group. The immunohistochemistry staining showed that the expression levels of osteoprotegerin and vascular endothelial growth factor in the experimental group were higher than those in the control group at each period (P < 0.05). Compared with the control group, expression peak of both osteoprotegerin and vascular endothelial growth factor have extended in the experimental group. This effectively shortened the time interval of both expression peak. Connective tissue growth factor can improve the expression levels of osteoprotegerin and vascular endothelial growth factor in callus tissue during fracture healing, and it can also promote the fracture healing by changing the expression peak of both osteoprotegerin and vascular endothelial growth facto

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