Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (45): 8520-8523.doi: 10.3969/j.issn.2095-4344.2012.45.030

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Wnt signaling pathway for osteogenic differentiation of bone marrow mesenchymal stem cells induced by eucommia bark

Zhang Xian1, Zhu Li-hua1, Qian Xiao-wei2, Tan Xiang-ling2   

  1. 1Wuxi Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Wuxi 214000, Jiangsu Province, China
    2College of Life Sciences, Nantong University, Nantong 226019, Jiangsu Province, China
  • Received:2012-01-09 Revised:2012-02-28 Online:2012-11-04 Published:2012-11-04
  • Contact: Tan Xiang-ling, Master, Professor, College of Life Sciences, Nantong University, Nantong 226019, Jiangsu Province, China tanxl@ntu.edu.cn
  • About author:Zhang Xian, Associate chief physician, Wuxi Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Wuxi 214000, Jiangsu Province, China zhangxian0772@sina.com

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Abstract:

BACKGROUND: Recently, there are many reports describing Chinese medicine and the effective components of Chinese medicine for treatment of osteoporosis. But there are few reports regarding cellular osteogenic differentiation and regulation signaling.
OBJECTIVE: To investigate the expression of Wnt signaling pathway related genes during osteogenic differentiation of rat bone marrow mesenchymal stem cells induced by eucommia bark.
METHODS: Passage 3 Sprague-Dawley rat bone marrow mesenchymal stem cells were inoculated into 6-well plate, with 1×103 cells per well. 24 hours later, induced medium supplemented with 7.5% fetal bovine serum-containing DMEM/F12 (1:1) and 1/1 000 eucommia bark was refreshed. Normal culture medium was used in the negative control group. After induction for 8 hours, 1, 3, and 7 days, expression levels of Fzd and LRP receptors of Wnt passway, β-catenin, intranuclear Wnt target genes and Wnt inhibitory factor were determined by RT-qPCR.
RESULTS AND CONCLUSION: Compared with negative control group, Fzd2 expression increased to 11.86 folds and Fzd3 expression increased to 2 folds respectively after induction for 3 days; Fzd2 expression increased to 5.12 folds and Fzd3 rebounded to normal level after induction for 7 days; β-catenin expression increased to 2 folds after induction for 3 days, and WIF1 expression decreased significantly after induction for 3 and 7 days, respectively. These findings suggest that Wnt signaling pathway may participate in the process of eucommia bark promoting in osteogenic differentiation of bone marrow mesenchymal stem cells.

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