Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (45): 8510-8513.doi: 10.3969/j.issn.2095-4344.2012.45.028

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Effects of X binding protein-1 gene-modified neural stem cells transplantation on expression of related neurotransmitters and nucleoprotein in the substantia nigra of rat Parkinson's disease model

Zhang Yan-hua1, Lu Lei2, Dong Jing1, Song Lei3, He Chun-ke4, Zhang Yuan5   

  1. 1Department of Neurology, Affiliated Hospital of Jilin Medical College, Jilin 132013, Jilin Province, China; 2Department of Neurology, Harrison International Peace Hospital, Hengshui 053000, Hebei Province, China; 3Department of Neurology, 5Department of Neonatology, Norman Bethune First Hospital of Jilin University, Changchun 130021, Jilin Province, China; 4Department of Orthopedics, Jilin Hospital of Integrated Traditional Chinese with Western Medicine, Changchun 130021, Jilin Province, China
  • Received:2012-02-19 Revised:2012-04-22 Online:2012-11-04 Published:2012-11-04
  • About author:Zhang Yan-hua, Attending physician, Department of Neurology, Affiliated Hospital of Jilin Medical College, Jilin 132013, Jilin Province, China neurology@139.com

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Abstract:

BACKGROUND: Previous studies have shown that X binding protein-1 gene can be transfected into the neural stem cells and is steadily expressed. X binding protein-1 gene transfected neural stem cells transplantation for cerebral infarction treatment is better than ordinary neural stem cells.
OBJECTIVE: To study the treatment effect of X binding protein-1 transfected neural stem cells transplantation on Parkinson's disease through detecting the influence on monoamine neurotransmitters and α-synuclein levels in the substantia nigra of rat Parkinson's disease model.
METHODS: Twenty-seven rat Parkinson's disease models were divided into three groups randomly, and 9 rats in each group. The rats in control group were transplanted with PBS through the lateral ventricle, while neural stem cells group was transplanted with blank neural stem cell suspension, and transfection group was transplanted with X binding protein-1 gene-modified neural stem cells. In 7, 14, 21 and 28 days after transplantation, neurobehavioral score of the rats was estimated respectively, α-synuclein level was examined by Western blot, and the tyrosine hydroxylase positive cells were revealed by immunohistochemical staining.
RESULTS AND CONCLUSION: The expression of α-synuclein was decreased in the transfection group. The number of tyrosine hydroxylase positive cells in the transfection group was higher than that in the neural stem cells group and control group, and the rotation speed of transfection group was decreased obviously. The results show that the differentiation rate of binding protein-1 gene-modified neural stem cells differentiating into dopaminergic neurons is higher and the transfection can reduce α-synuclein content in rat substantia nigra, so as to treat Parkinson's symptoms.

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