Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (45): 8424-8429.doi: 10.3969/j.issn.2095-4344.2012.45.012

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Construction of vascularized adipose using adipose-derived stem cells and autogeneic platelet-rich plasma carrier complex in vivo by tissue engineering

Li Hong-mian1, 2, Liu Da-lie3, Zhao Pei-ran4, Liang Shuang-wu4   

  1. 1Postdoctoral Research Center, Department of Plastic and Aesthetic Surgery, the Affiliated Zhongshan Hospital of Sun Yat-Sen University, Zhongshan 528403, Guangdong Province, China
    2Postdoctoral Research Center, Southern Medical University, Guangzhou 510515, Guangdong Province, China
    3Department of Plastic and Reconstructive Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou 501282, Guangdong Province, China
    4Research Center for Tissue Engineering, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • Received:2012-01-02 Revised:2012-01-09 Online:2012-11-04 Published:2012-11-04
  • Contact: Liu Da-lie, Professor, Chief physician, Doctoral supervisor, Department of Plastic and Reconstructive Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou 501282, Guangdong Province, China Liudalie@hotmail.com
  • About author:Li Hong-mian☆, Doctor, Associate chief physician, Master’s supervisor, Postdoctoral Research Center, Department of Plastic and Aesthetic Surgery, the Affiliated Zhongshan Hospital of Sun Yat-Sen University, Zhongshan 528403, Guangdong Province, China binrong2112@163.com

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Abstract:

BACKGROUND: Revascularization mechanism is a determinal factor of successful construction of adipose tissue by tissue engineering.
OBJECTIVE: To investigate the feasibility of construction of vasuclarized adipose using adipose-derived stem cells and autogeneic platelet-rich plasma carrier complex in vivo by tissue engineering。
METHODS: Adipose-derived stem cells were isolated from the subcutaneous adipose tissue of healthy adult after liposuction, and primary culture and subculture of adipose-derived stem cells were conducted. After being induced towards adipocytes for 2 weeks, 5× 1010/L passage 3 cell suspension labeled by BrdU was prepared. Two groups were included: experimental group, in which 0.5 mL cell suspension, 100 μL platelet-rich plasma and 0.5 mL fibrin glue were implanted into the subcutaneous fascia of nude mice; control group in which 0.5 mL cell suspension, 100 μL DMEM and 0.5 mL fibrin glue were implanted into the subcutaneous fascia of nude mice.
RESULTS AND CONCLUSION: At 8 weeks after surgery, neogenetic vessels grew into the scaffolds and mild fiber encapsulation was observed in the experimental group, while few vessels grew into the scaffolds and mild fiber encapsulation was also observed in the control group. The wet weight of cambium in the experimental group was higher than that in the control group (P < 0.01). Hematoxylin-eosin staining showed the formation of neogenetic adipose tissues and the growth of micrangium in the implant. The number of micro vessels in the experimental group was greater than that in the control group (P < 0.01). The immunofluorescence staining of cambium showed that the cell nucleus of regenerated adipocytes and partial capillary endothelium in both groups presented green fluorescence. It is feasible to prepare vasuclarized adipose using adipose-derived stem cells and autogeneic platelet-rich plasma carrier complex in vivo by tissue engineering. Adipose-derived stem cells and autogeneic platelet-rich plasma participate in neovascularization of neogenetic adipose tissue. The autogeneic platelet-rich plasma can promote revascularization of tissue-engineered complex and ensure survival of more seed cells.

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