Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (45): 8419-8423.doi: 10.3969/j.issn.2095-4344.2012.45.011

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Bone morphogenetic protein 2 expression in umbilical cord mesenchymal stem cells after adipogenic induction

Chi Ying, Rong Li-juan, Xu Fang-yun, Han Zhi-bo, Yang Shao-guang, Wang You-wei, Ma Feng-xia, Lu Shi-hong, Han Zhong-chao   

  1. State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China
  • Received:2012-01-26 Revised:2012-03-03 Online:2012-11-04 Published:2012-11-04
  • Contact: Han Zhong-chao, Doctor, Professor, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China hanzhongchao@ hotmail.com
  • About author:Chi Ying★, Master, Associate professor, State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China caizhuying@ 126.com

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Abstract:

BACKGROUND: Expression of bone morphogenetic protein 2 and peroxisome proliferator-activated receptorγgene in umbilical cord mesenchymal stem cells after adipogenic induction is increased at the same time.
OBEJECTIVE: To investigate the changes of bone morphogenetic protein 2 and peroxisome proliferator-activated receptorγgene expression in umbilical cord mesenchymal stem cells after adipogenic induction, and to compare the results.
METHODS: With specific inductive medium, umbilical cord mesenchymal stem cells were obtained and then induced into adipogenisis. Their morphological characteristics were observed with inverted microscope. Oil red O staining was used to observe the adipogenic phenomenon, and von kossa staining and alizarin red staining were used to identify the formation of precipitation. Real-time polymerase chain reaction was used to quantify the gene expression of bone morphogenetic protein 2 and peroxisome proliferator-activated receptor γ at various time points after adipogenic induction.
RESULTS AND CONCLUSION: Umbilical cord mesenchymal stem cells could be isolated and cultured successfully. After cultured in adipogenic medium, the cells differentiated into adipogenisis. The result of real-time polymerase chain reaction showed that both the expression levels of bone morphogenetic protein 2 and peroxisome proliferator-activated receptorγgene were increased after adipogenic induction, but peroxisome proliferator-activated receptorγplayed a major role in the regulation of differentiation.

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