Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) can differentiate into the native cells of renal tissue to repair the injured renal tissue. Under normal circumstance, peripheral blood stem cells are limited in number, and mobilization of BMSCs can increase the number of peripheral blood stem cells.
OBJECTIVE: To observe the therapeutic effects of BMSC mobilization on repair of ischemia/reperfusion-induced renal injury and on hypoxia inducible factor-1a and to investigate the mechanism by which BMSC mobilization repairs renal injury.
METHODS: Sprague-Dawley rats were randomly allocated into four groups: In the control group, there was no treatment. In the model group, renal ischemia/reperfusion model was prepared. In the treatment group, 200 μg/kg per day stem cells and 50 μg/kg per day granulocyte colony-stimulating factors were subcutaneously administered in rat models of ischemia/reperfusion renal injury to mobilize BMSCs. In the treatment control group, normal rats received the same administration as rats in the treatment group. Drug administration was performed for a total of 5 successive days. At 5, 10, 17, 24, 31 days post-surgery, renal tissue was resected for pathological observation, and the expression level of CD34⁺ cells, hypoxia inducible factor-1a, vascular endothelial growth factor and heme oxygenase 1 was detected.
RESULTS AND CONCLUSION: Application of stem cell factors combined with granulocyte colony-stimulating factors could significantly increase BMSCs in the injured renal tissue and alleviate the injury degree of renal tissue. BMSCs can increase hypoxia inducible factor-1a expression, vascular endothelial growth factor and heme oxygenase 1, which may be one of possible mechanisms by which BMSCs promote the repair of acute renal injury. BMSC mobilization can promote the expression of hypoxia inducible factor-1a system.