Abstract:

BACKGROUND: Trachea substitutes include autologous tissue flap, allogeneic tracheal transplant, artificial scaffolds and non-dynamic tissue transplant. However, they are hardly used in the clinic because of severe complications and difficult harvest.
OBJECTIVE: To establish the tissue-engineered tracheal scaffold model by adipose-derived stem cells, poly-D,L-lactio-co-glycolic acid and poly(trimethylenecarbonate).
METHODS: Adipose-derived stem cells of Sprague Dawley rats were isolated and cultured by tissue block method. Cell surface markers were evaluated by flow cytometry and multilineage differentiation, and the cells were implanted on the poly-D,L-lactio-co-glycolic-poly(trimethylenecarbonate) scaffold. After in vitro and in vivo culture, the adipose-derived stem cells were observed by immunohistochemistry and scanning electron microscope.
RESULT AND CONCLUSION: Adipose-derived stem cells were spherical and well attached to the scaffolds with lamellipodia and conglobate connection after inoculated on the poly-D,L-lactio-co-glycolic-poly(trimethylenecarbonate) scaffold. After in vitro culture, the newborn capillary could be seen. The poly-D,L-lactio-co-glycolic- poly(trimethylenecarbonate) scaffold scaffolds with porous structure were highly biocompatible and non-cytotoxic as determined by adipose-derived stem cells adhesion and proliferation. Neotrachea fabricated with combined in vitro and in vivo culture system using poly-D,L-lactio-co-glycolic-poly(trimethylenecarbonate) scaffold and adipose-derived stem cells can meet the requirement of tracheal replacement, this model serve as a new way to engineer a vascularized trachea.