Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (41): 7651-7657.doi: 10.3969/j.issn.2095-4344.2012.41.011

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Effect of adipose-derived stem cells modified with hypoxia-inducible factor-1 alpha gene on acute renal injury induced by cisplatin in mice

Wang Wei-wei, Jiang Yan, Wang Wei, Cheng Jin, Zhang Jin-yuan   

  1. Department of Nephrology, the 455 Hospital of Chinese PLA, Nephrology Institutes of Nanjing Military Area, Shanghai 200052, China
  • Received:2012-07-29 Revised:2012-08-28 Online:2012-10-07 Published:2012-10-07
  • Contact: Zhang Jin-yuan, Chief physician, Professor, Doctoral supervisor, Department of Nephrology, the 455 Hospital of Chinese PLA, Nephrology Institutes of Nanjing Military Area, Shanghai 200052, China jinyuan_zhang@163.com
  • About author:Wang Wei-wei☆, Doctor, Associate chief physician, Master’s supervisor, Department of Nephrology, the 455 Hospital of Chinese PLA, Nephrology Institutes of Nanjing Military Area, Shanghai 200052, China w.vwei@163.com

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Abstract:

BACKGROUND: Stem cells transplantation provides a new way for the treatment of renal injury. The effect could be enhanced by transfecting target gene before transplantation.
OBJECTIVE: To explore the effect of adipose-derived mesenchymal stem cells transfected with hypoxia-inducible factor-1α gene on the structure and function of the kidney in mice with acute renal injury induced by cisplatin.
METHODS: The cisplatin-induced acute renal injury mice model was established by intraperitoneal injecting with 10 mg/kg cisplatin for 2 days. At 24 hours after modeling, adipose-derived mesenchymal stem cells suspension containing 1×105 adipose-derived mesenchymal stem cells or hypoxia-inducible factor-1α was administrated through tail vein, after 3 days, the blood and kidney samples were detected. The acute renal injury mice injected with 200 μL normal saline were regarded as model controls, and the normal mice were as normal controls.
RESULTS AND CONCLUSION: After adipose-derived mesenchymal stem cells intervention, the serum creatinine and blood urea nitrogen level were decreased, and the pathological changes of renal tissues and apoptosis lesions of renal tubular epithelial cells were decreased, as well as the expression of inflammatory cytokines RANTES of kidney tissue and tumor necrosis factor-α, while the expression of interleukin-10 was increased; the hypoxia-inducible factor-1α modified adipose-derived mesenchymal stem cells had a positive effect on renal cell apoptosis and expression of inflammatory cytokines. The immunofluorescence staining showed that a small quantity of green fluorescent protein from adipose-derived mesenchymal stem cells was present in renal tissue, but transformatation to the renal tubular epithelial cells was not observed. The results show that adipose-derived mesenchymal stem cells transplantation can improve the damage of the kidney structure and function in acute renal injury, and the adipose-derived mesenchymal stem cells transfected with hypoxia-inducible factor-1α are more effective in repairing acute renal injury.

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