Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (41): 7647-7650.doi: 10.3969/j.issn.2095-4344.2012.41.010

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Temporal specificity of differentiation of rat embryonic neural stem cells cultured in vitro

Huang Fei, Zhao Ji-tong, Shen Qiang   

  1. Department of Orthopedics, Shanghai First People’s Hospital of Shanghai Jiao Tong University, Shanghai 200080, China
  • Received:2012-02-09 Revised:2012-03-27 Online:2012-10-07 Published:2012-10-07
  • Contact: Shen Qiang, M.D., Chief physician, Professor, Master’s supervisor, Department of Orthopedics, Shanghai First People’s Hospital of Shanghai Jiao Tong University, Shanghai 200080, China shmail1231@yahoo.com
  • About author:Huang Fei★, Studying for master’s degree, Department of Orthopedics, Shanghai First People’s Hospital of Shanghai Jiao Tong University, Shanghai 200080, China huangfei3738@126.com

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Abstract:

BACKGROUND: There have been few studies describing the characteristics of self differentiation of neural stem cells cultured in vitro.
OBJECTIVE: To investigate the temporal specificity of differentiation of rat embryonic neural stem cells cultured in vitro.
METHODS: Neural stem cells were isolated from rat embryonic cerebral cortex and cultured in serum-free medium. Neural stem cells cultured for different time were induced to differentiate and the change of differentiation properties was detected.
RESULTS AND CONCLUSION: Neural stem cells cultured in vitro were induced to differentiate predominately into neurons at early phase. As the increase of incubation time, the proportion of neural stem cells differentiating into neurons was decreased, and the proportion of neural stem cells differentiating into astrocytes was increased. However, there was no obvious correlation between the differentiation potential of neural stem cells into oligodendrocytes and incubation time. These findings suggest that as the increase of incubation time, the differentiation potential of neural stem cells into neurons was reduced and the differentiation potential of neural stem cells into astrocytes was enhanced; there was no obvious correlation between the differentiation potential of neural stem cells into oligodendrocytes and incubation time.

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