Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (41): 7637-7641.doi: 10.3969/j.issn.2095-4344.2012.41.008

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Effect of platelet lysate on osteogenic differentiation of human umbilical cord derived mesenchymal stem cells in vitro

Lü Jiang-tao1, Tian Shao-qi1, Sun Kang1, Zhang Ji-hua2, Zhang Ying-ying1, Zhang Cai-long1, Liu Shi-hai3, Feng Xue-tao1   

  1. 1Department of Orthopedics, 2Regular Physical Examination Center, 3Central Lab, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China
  • Received:2012-05-06 Revised:2012-07-25 Online:2012-10-07 Published:2012-10-07
  • Contact: Sun Kang, Professor, Master’s supervisor, Doctoral supervisor, Department of Orthopedics, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China sunkang_qy@yahoo.com.cn
  • About author:Lü Jiang-tao★, Studying for master’s degree, Department of Orthopedics, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China lvjiangtao87@163.com

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Abstract:

BACKGROUND: Platelet lysate is the pyrolysis products of concentrated platelet which contains many active ingredients. Studies have shown that the active ingredients can promote the proliferation and differentiation of mesenchymal stem cells, but the effect of platelet lysate on the osteogenic differentiation of mesenchymal stem cells is not clear.
OBJECTIVE: To study the intervention effect of platelet lysate in osteogenic differentiation of human umbilical cord derived mesenchymal stem cells in vitro.
METHODS: Human umbilical cord derived mesenchymal stem cells isolated by collagenase digesting were cultured and amplified in vitro. Flow cytometer was used to detect the cell surface markers. The experiment was divided into four groups: mineralization medium group, platelet lysate group, mineralization medium-platelet lysate group and control group. The cells were cultured with corresponding induction medium for 2 weeks, then alizarin red staining was used to identify the oxteoblast, and alkaline phosphatase activity was used to compare the differentiation activity.
RESULTS AND CONCLUSION: Flow cytometer results showed that CD34, CD45 and HLA-DR were negative in the human umbilical cord derived mesenchymal stem cells while CD44, CD105 and CD146 were positive. Alizarin red staining was positive in three induced groups with no obvious differences, and it was negative in the control group. The alkaline phosphatase activity level in three induced groups was significantly higher than that in the control group (P < 0.05), and the alkaline phosphatase activity level in mineralization medium-platelet lysate group was significantly higher than that in the mineralization medium group and platelet lysate group (P < 0.05). Results show that 5% platelet lysate alone can induce human umbilical cord derived mesenchymal stem cells to differentiate into osteoblasts in vitro. The induction will be more efficient when combining 5% platelet lysate with mineralization medium.

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