Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (32): 5909-5914.doi: 10.3969/j.issn.2095-4344.2012.32.004

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In vivo and in vitro tracking of bone marrow mesenchymal stem cells delivered to infarcted myocardium by superparamagnetic iron oxide nanoparticles

Adila·Azat, Ma Xiang, Chen Bang-dang, Liu Fen, Ma Yi-tong   

  1. Cardiac Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Received:2012-01-09 Revised:2012-02-08 Online:2012-08-05 Published:2012-08-05
  • Contact: Ma Yi-tong, Professor, Chief physician, Doctoral supervisor, Cardiac Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • About author:Adila·Azat☆, Ph.D., Associate chief physician, Cardiac Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China 1057038365@ qq.com

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Abstract:

BACKGROUND: In vivo tracking of transplanted stem cells and evaluating the dynamic changes of transplanted stem cells by imageological means is currently a rapidly growing area of research.
OBJECTIVE: To investigate the feasibility of in vivo and in vitro tracking of mesenchymal stem cells delivered to infarcted myocardium by surpurparamagnetic iron oxide nanoparticles.
METHODS: Eight pig models of acute myocardial infarction were successfully established by occlusion of anterior descending coronary artery with balloon and were then randomly divided into two groups. In the experimental group, at 2 weeks after acute myocardial infarction induction, 1 mL bone marrow mesenchymal stem cells labeled by the mixture of surpurparamagnetic iron oxide nanoparticles and polylysine were injected via five points in the center and border of infracted region. Equal amounts of physiological saline were identically injected in the control group.
RESULTS AND CONCLUSION: At 1 hour, 2 weeks after cell transplantation, MRI images of experiment group showed obvious low signal of transplantation region in all four pigs. At 4 weeks after transplantation, desmin, connexin and transcription protein were detected by fluorescent immunohistochemical staining. In vivo tracking of surpurparamagnetic iron oxide nanoparticles-labeled bone marrow mesenchymal stem cells by MRI is feasible and effective.

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