Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (32): 5903-5908.doi: 10.3969/j.issn.2095-4344.2012.32.003

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Stromal cell-derived factor-1 preconditioning inhibits apoptosis of rat bone marrow mesenchymal stem cells

Shu Bo, Liu Zhi-jiang, Fan Fang   

  1. 1Department of Biochemistry and Molecular Biology of Zunyi Medical College, Zunyi 563003, Guizhou Province, China;
    2Department of Cardiology, First Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China
  • Received:2012-01-19 Revised:2012-02-19 Online:2012-08-05 Published:2012-08-05
  • About author:Shu Bo★, Master, Lecturer, Department of Biochemistry and Molecular Biology of Zunyi Medical College, Zunyi 563003, Guizhou Province, China shubo1014@126.com

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Abstract:

BACKGROUND: Studies demonstrated that stromal cell-derived factor-1 may participate in anti-apoptosis of mesenchymal stem cells in addition to directed migration.
OBJECTIVE: To investigate that stromal cell-derived factor-1 preconditioning inhibits apoptosis of MSCs.
METHODS: Apoptosis of rat bone marrow mesenchymal stem cells was induced with different concentrations of H2O2. H2O2 at the optimal concentration of 100 μmol/L was used for experiments. Rat bone marrow mesenchymal stem cells were induced with 100 μmol/L H2O2 and then treated with stromal cell-derived factor-1 at different concentrations, and stromal cell-derived factor-1 at the optimal concentration was used for experiment. Passage 3 rat bone marrow mesenchymal stem cells were randomly divided into four groups. The normal control group was treated with nothing. The injured group was treated with 100 μM H2O2 for 24 hours. The stromal cell-derived factor-1 preconditioning group was treated with stromal cell-derived factor-1 at 6 hours before H2O2 addition. The stromal cell-derived factor-1+AMD3100 (an antagonist of stromal cell-derived factor-1 receptor CXCR4) group was incubated with stromal cell-derived factor-1 and AMD3100 at 6 hours before H2O2 addition.
RESULTS AND CONCLUSION: H2O2 could mimic ischemic environment and induce the apoptosis of bone marrow mesenchymal stem cells in a dose-dependent manner in vitro. After stromal cell-derived factor-1 preconditioning, cell apoptosis was significantly alleviated (P < 0.01), E2F6 and E2F1 mRNA expression was significantly increased (P < 0.05), cytochrome c translocation was significantly reduced (P < 0.05), caspase-3 activity was decreased (P < 0.05) compared with the injured group. AMD3100 could block the protective effect of stromal cell-derived factor-1 on bone marrow mesenchymal stem cells. These results suggest that stromal cell-derived factor-1 is capable to reduce the apoptosis of bone marrow mesenchymal stem cells by upregulating E2F6 mRNA expression, negatively regulating E2F1 mRNA expression, and decreasing cytochrome c release.

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