Increased expression of long-chain non-coding RNA LINC00511 in periodontitis promotes osteoclast proliferation

doi:10.3969/j.issn.2095-4344.1832

Abstract

Abstract:

BACKGROUND: LINC00511 is a relatively new long-chain non-coding RNA (Lnc RNA). Studies have shown that lincRNA-ANRIL is closely related to the occurrence and development of periodontal disease.
OBJECTIVE: To study the expression of LINC00511 in periodontitis, and to explore LINC00511 effects on osteoblast proliferation and differentiation.
METHODS: The study protocol was approved by the Ethics Committee of Stomatology Hospital of Shenyang. Patients and their relatives were fully informed of study protocol and informed consent was signed prior to the inception of the trial. Periodontal ligament tissues were extracted from patients with periodontitis and patients undergoing orthodontic extraction (control group). The expression of LINC00511, tartrate-resistant acid phosphatase and cathepsin K in periodontitis patients was detected by real-time PCR. The third generation of osteoclast precursor cells RAW264.7 were induced to differentiate into osteoclasts and identified. LINC00511-siRNA was transfected into osteoclasts, and non-transfected osteoclasts were used as control. Transfection efficiency was detected by real-time PCR. RAW264.7 cells were cultured with 100 μg/L lipopolysaccharide for 0, 24, and 48 hours, and the expression of LINC00511 was detected. After LINC00511 transfection, proliferation and differentiation of osteoclasts were detected by cell counting kit-8 and tartrate-resistant acid phosphatase, respectively.
RESULTS AND CONCLUSION: The levels of LINC00511, tartrate-resistant acid phosphatase and cathepsin K in human periodontitis samples were markedly higher than those in the control group. The expression of LINC00511 was time-dependently increased in osteoclasts after lipopolysaccharide treatment. Findings from fluorescence microscopy and real-time PCR showed that the expression of LINC00511 was significantly decreased after LINC00511-siRNA transfection compared with the control group (P < 0.05). LINC00511 promoted the proliferation and differentiation of osteoclasts. To conclude, the expression of LINC00511 is increased in periodontitis samples, and LINC00511 can promote the proliferation of osteoclasts.

Key words: osteoclasts, periodontitis, RAW264.7 cells, proliferation, LINC00511, Lnc RNA

CLC Number:

Zhao Xiangyu, Zhang Guirong, Guo Chuanbo, Shi Chun, Wu Liuzhong. Increased expression of long-chain non-coding RNA LINC00511 in periodontitis promotes osteoclast proliferation[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(33): 5360-5365.

Figures/Tables 3

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