Mogroside V promotes bone formation by stimulating osteoblast proliferation and differentiation

doi:10.3969/j.issn.2095-4344.1798

Abstract

Abstract:

BACKGROUND: Bone remodeling is going on throughout the lifetime. Bone homeostasis is a dynamic balance between bone formation of osteoblasts and bone resorption of osteoclasts. Controlling of bone remodeling at the cellular level is critical to maintaining bone homeostasis in response to structural and metabolic demands.
OBJECTIVE: To explore the effect of mogroside V on the proliferation and differentiation of osteoblasts.
METHODS: Rat osteoblasts were extracted by two-step enzymatic digestion, followed by MTT assay to detect the toxicity of mogroside V. The proliferation of osteoblasts was observed by FDA/PI fluorescence staining at 2, 4 and 6 days after the treatment with mogroside V (0, 6.25×10^-3, 1.25×10^-2, 2.5×10^-2 g/L). After 6 days of treatment on osteoblasts, the effect of mogroside V on osteoblast differentiation was studied by alkaline phosphatase staining, alizarin red staining and qRT-PCR.
RESULTS AND CONCLUSION: MTT assay showed that the mogroside V at 6.25×10^-3 to 2.5×10^-2 g/L promoted osteoblast proliferation. FDA/PI fluorescence staining showed that mogroside V (6.25×10^-3, 1.25×10^-2, 2.5×10^-2 g/L) could improve osteoblast activity and promote cell proliferation, especially when the concentration of mogroside V was 1.25×10^-2 g/L. After 6 days of treatment, alkaline phosphatase staining and alizarin red staining showed that the mogroside V, especially at 1.25×10^-2 g/L, promoted osteogenesis and mineralization. qRT-PCR results showed that the expressions of runt related transcription factor 2, bone sialoprotein, osteocalcin and alpha-1 type I collagen genes were higher in the mogroside V group than the blank group, especially when the concentration of mogroside V was 1.25×10^-2g/L. Therefore, mogroside V, especially at 1.25×10^-2 g/L, promotes bone formation by stimulating osteoblast proliferation and differentiation.

Key words: mogroside V, osteoblasts, cell proliferation, cell differentiation, bone formation, osteoporosis, Guangxi Natural Science Foundation

CLC Number:

Yao Shunhan, Liao Liang, Qin Jiagang, Wei Huacheng. Mogroside V promotes bone formation by stimulating osteoblast proliferation and differentiation[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(29): 4701-4706.

Figures/Tables 3

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