Bone marrow mesenchymal stem cell transplantation combined with Lindera glauca leaf extract attenutes inflammation after spinal cord injury

doi:10.3969/j.issn.2095-4344.1681

Abstract

Abstract:

BACKGROUND: Studies have shown that bone marrow mesenchymal stem cells have a high value in the early treatment of spinal cord injury. Lindera glauca leaves rich in flavonoids, saponins and other substances can scavenge free radicals and suppress lipid peroxidation. However, the combination treatment of bone marrow mesenchymal stem cells and Lindera glauca leaf extract in the spinal cord injury model is still rare.
OBJECTIVE: To observe the effect and mechanism of bone marrow mesenchymal stem cell transplantation combined with Lindera glauca leaf extract on spinal cord injury.
METHODS: Fifty-two adult Sprague-Dawley rats were randomly divided into sham operation (n=12), spinal cord injury (n=14), cell transplantation (n=13) and combination treatment (n=13) groups. The spinal cord injury model was established by the Allen’s method in the rats of spinal cord injury, cell transplantation and combination treatment groups. In the sham operation group, the spinal cord was only exposed but not subjected to the Allen’s striking method. The rats in the cell transplantation and combination treatment groups were administered bone marrow mesenchymal stem cells (1.5×10⁵) 0.5 mm away from the midline of the injured spinal cord. Moreover, the rats in the combination treatment group were then intragastrically given 5 mg/kg Lindera glauca leaf extract, once a day for 7 continuous days. At 1, 5, 7 days after model establishment, 4 rats from each group were used for Basso, Beattie and Bresnahan score and spinal cord cell apoptosis counting, and the expression of tumor necrosis factor-α in the spinal cord was detected by immunohistochemistry.
RESULTS AND CONCLUSION: (1) Compared with the spinal cord injury group, the hindlimb motor function was significantly restored in the rats of cell transplantation and combination treatment groups. The Basso, Beattie and Bresnahan scores in the cell transplantation and combination treatment groups were markedly higher than those in the spinal cord injury group at 5 and 7 days after modeling (P < 0.05 or P < 0.01). And the Basso, Beattie and Bresnahan scores in the combination treatment group were higher than those in the cell transplantation group (P < 0.05). (2) After treatment, the rats in the combination treatment group exhibited significantly reduced signs of tissue edema and necrosis at the injury site, in which the number of apoptotic cells and the expression of tumor necrosis factor α were significantly decreased. All the sighs in the combination treatment group were improved as compared with the cell transplantation group. These findings indicate that bone marrow mesenchymal stem cell transplantation combined with Lindera glauca leaf extract can treat the spinal cord injury effectively, and its effect may be related to the reduction of tumor necrosis factor α-mediated inflammatory response.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Lindera, Spinal Cord Injuries, Tumor Necrosis Factor-alpha, Tissue Engineering

CLC Number:

Cheng Jianping, Li Hua, Li Xiongjie. Bone marrow mesenchymal stem cell transplantation combined with Lindera glauca leaf extract attenutes inflammation after spinal cord injury[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(13): 1975-1981.

Figures/Tables 3

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