Bone marrow mesenchymal stem cell conditioned medium with inflammatory activation for repair of radiation-induced IEC-6 cell injury

doi:10.3969/j.issn.2095-4344.1584

Abstract

Abstract:

BACKGROUND: Conditioned medium from mesenchymal stem cells (MSC-CM) may represent a promising alternative to MSCs transplantation. Our previous study has demonstrated that MSC-CM with inflammatory activation improves the structural and functional restoration of the small intestine after radiation-induced intestinal injury, improve the survival status of rats with acute radiation injury, but its potential cellular mechanism has not been further explored.
OBJECTIVE: To observe the effect of MSC-CM with inflammatory activation on the proliferation and apoptosis of intestinal epithelial cells (IEC-6) after radiation injury and to investigate the cellular mechanism of pre-activated MSC-CM in repairing the small intestinal mucosa.
METHODS: IEC-6 cells were divided into four groups: control group, radiation injury group, normal MSC-CM (MSC-CM^IEC-6(NOR)) group and inflammatory pre-activated MSC-CM (MSC-CM^IEC-6(IR)) group. IEC-6 cells in the latter three groups were exposed to 10 Gy X-ray irradiation and cultured in DMEM-F12 medium, MSC-CM^IEC-6(IR) and MSC-CM^IEC-6(NOR) respectively. Cells in the control group were only cultured in DMEM-12 medium. Cultured cells were collected at 3 days after radiation to observe the proliferation of IEC-6 cells by using proliferating cell nuclear antigen immunofluorescence staining, and to observe the apoptosis by using TUNEL apoptosis staining and western blot assay.
RESULTS AND CONCLUSION: Compared with the radiation injury group, in the MSC-CM^IEC-6(IR) group, the number of cells positive for proliferating cell nuclear antigen increased significantly (P < 0. 05), the number of TUNEL positive cells decreased significantly (P < 0. 05), and the expression of Caspases-3 decreased significantly (P < 0.05), However, there was no significant difference between the MSC-CM^IEC-6(NOR) group and radiation injury group (P > 0.05). Taken together, MSC-CM^IEC-6(IR), but not non-activated MSC-CM, significantly promotes the proliferation and reduces apoptosis of intestinal epithelial cells after radiation injury and therefore repair the injured intestinal tissue.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Mesenchymal Stem Cells, Culture Media, Conditioned, Intestine, Small, Radiation Injuries, Tissue Engineering

CLC Number:

Li Jinliang, Luo Yujun, Ma Faxin, Zheng Yue, Lu Quan, Zhou Weijie, Zhang Chen, Sha Weihong, Chen Hao. Bone marrow mesenchymal stem cell conditioned medium with inflammatory activation for repair of radiation-induced IEC-6 cell injury[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(5): 697-702.

Figures/Tables 1

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