Effects of normoxic and hypoxic recovery on rat skeletal muscle focal adhesion complex after a single bout eccentric exercise

doi:10.3969/j.issn.2095-4344.1483

Abstract

Abstract:

BACKGROUND: Excessive training or non-adaptive high-intensity exercise makes negative effect on the skeletal muscle, and causes changes in ultrastructure of muscle fibers, thus causing damage. Exercise-induced mechanical stimuli can regulate gene expression by physical connection between cytoskeleton and nuclear matrix.
OBJECTIVE: To investigate the effects of different recovery time and recovery conditions on the protein content of skeletal muscle focal adhesion complex after one eccentric exercise.
METHODS: The study was approved by the Experimental Animal Ethics Committee of Beijing Sport University, approval number: 2014011. Fifty-six male Sprague-Dawley rats aged 8 weeks were randomly divided into control group, normoxic recovery group and hypoxic recovery group. Normoxic recovery group was subjected to a bout high-intensity eccentric exercise and hypoxia treatment (12.7% O2) while hypoxic recovery group performed exercise intervention. Hypoxic recovery and normoxic recovery groups were divided into 24-, 48- and 72-hour groups according to different time points after exercise. The gastrocnemius muscle was taken at the corresponding time phase. The expression levels of integrin α7 and β1, p-FAK and p-paxillin protein were detected by western blot assay.
RESULTS AND CONCLUSION: (1) Compared with the control group, the expression level of integrin α7 in the 24-hour normoxic recovery group was significantly increased (P < 0.05), and restored to the level of control group with time. The expression level of integrin α7 in the 72-hour hypoxic recovery group was higher than that in the control group (P < 0.01). (2) The expression level of integrin β1 in the normoxic recovery and hypoxic recovery groups was on a rise, and the expression level in the normoxic recovery group was higher than that in the hypoxic recovery group. The expression level in the 48- and 72-hour normoxic recovery groups was significantly higher than that in the control group (P < 0.05, P < 0.01). (3) The expression level of p-FAK in the normoxic recovery and hypoxic recovery groups was on a descent, and the expression level in the normoxic recovery group was higher than that in the hypoxic recovery group. The expression level in the 48- and 72-hour normoxic recovery groups was significantly lower than that in the control group (P < 0.05). (4) The expression level of p-paxillin in the normoxic recovery and hypoxic recovery groups was firstly decreased and then increased, and the expression level in each group except for 24-hour normoxic recovery group was significantly lower than that in the control group (P < 0.05). (5) In summary, a single bout eccentric exercise can affect the protein expression of skeletal muscle focal adhesion complex. Different recovery conditions have different effects on focal adhesion complex. Normal oxygen is beneficial to the recovery of integrin α7, while hypoxia is beneficial to integrin β1, p-FAK and p-paxillin.

Key words: skeletal muscle, hypoxia, exercise, focal adhesion complex, integrin, focal adhesion kinase, paxillin

CLC Number:

Chen Shengju, Wang Danni, Wang Jingtong, Yang Ning, Lei Binkai, Li Junping . Effects of normoxic and hypoxic recovery on rat skeletal muscle focal adhesion complex after a single bout eccentric exercise [J]. Chinese Journal of Tissue Engineering Research, 2019, 23(31): 4956-4961.

Figures/Tables 4

References

[1]王瑞元,周越.骨骼肌与运动[M].北京:人民体育出版社,2013.

[2]Armstrong RB,Warren GL,Warren JA.Mechanisms of exercise-induced muscle fiber injury.Sports Med. 1991;12(3):184-207.

[3]Armstrong RB.Mechanisms of exercise-induced delayed onset muscle soreness: a brief review.Med Sci Sport Exerc.1984;16(6):529-538.

[4]Barash IA, Peters D, Friden J, et al. Desmin cytoskeletal modification after a bout of eccentric exercise in the rat. Am J Physiol Regul Inter Comp Physiol.2002;283(4):R958-R963.

[5]马新东,高前进,李俊平.离心运动对细胞外基质蛋白含量及膜完整性的影响[J].北京体育大学学报, 2008, 31(5): 618-622.

[6]Bao ZZ,Lakonishok M,Kaufman S,et al.Alpha7 beta1 integrin is a component of the myotendinous junction on skeletal muscle.J Cell Sci.1993;106(Pt2): 579-589.

[7]Boppart MD, Burkin DJ, Kaufman SJ. Alpha7beta1-integrin regulates mechanotransduction and prevents skeletal muscle injury.Am J Physiol Cell Physiol.2006;290(6): C1660-1665.

[8]Kaariainen M, Nissinen L, Kaufman S, et al. Expression of alpha7 beta1 integrin splicing variants during skeletal muscle regeneration. Am J Pathol.2002;161(3): 1023-1031.

[9]蒋晓萍.低氧?低氧运动对大鼠骨骼肌黏着斑复合体的影响[D].北京:北京体育大学,2008.

[10]Armstrong RB, Ogilvie RW, Schwane JA. Eccentric exercise-induced injury to rat skeletal muscle. J Appl Physiol Respir Environ Exerc Physiol. 1983;54(1):80-93.

[11]Pica A, Brooks GA. Effects of training and age on VO2max in laboratory rats.Med Sci Sports Exerc.1982; 14(3): 249-252.

[12]Wong VW,Akaishi S,Longaker MT,et al.Pushing back: would mechanotransduction in repair and regeneration.J Invest Dermatol. 2011;131(11): 2186-2196.

[13]Lamon S,Wallace MA,Stefanetti RJ,et al.Regulation of the STARS signaling pathway in response to endurance and resistance exercise and training.Pflugers Arch.2013;465(9): 1317-1325.

[14]Emil R,Kristian V.Mechanosensitive molecular networks involved in transducing resistance exercise-signals into muscle protein accretion. Front Physiol.2016;7: 547.

[15]Chen HC,Guan JL.Association of focal adhesion kinase with its substrate phosphatidylinositol kinase.Proc Nat Acad Sci USA.1994;91(21): 10148-10152.

[16]Burkin DJ,Wallace GQ,Nicol KJ,et al.Enhanced expression of the alpha 7 beta 1 integrin reduces muscular dystrophy and restores viability in dystrophic mice.J Cell Biol.2001;152(6): 1207-1218.

[17]Liu J,Milner DJ,Boppart MD,et al.β1D chain increases α7β1 integrin and laminin and protects against sarcolemmal damage in mdx mice.Hum Mol Genet.2012;21(7): 1592-1603.

[18]Lueders TN,Zou K,Huntsman HD,et al.The α7β1-integrin accelerates fiber hypertrophy and myogenesis following a single bout of eccentric exercise. Am J Phsiol Cell Physiol.2011;301(4): C938-946.

[19]Graham ZA,Touchberry CD,Gupte AA,et al.Changes in α7β1 integrin signaling after eccentric exercise in heat-shocked rat soleus.Muscle Nerve.2015;51(4): 562-568.

[20]Rintanen N,Karjalainen M,Alanko J,et al. Calpains promote α2β1 integrin turnover in nonrecycling integrin pathway.Mol Biol Cell. 2012;23(3):448-463.

[21]Zhang F, Guo F. Effect of transplantation of cardiac stem cells overexpressing integrin?linked kinase on cardiac function of rats with acute myocardial infarction. Exp Ther Med.2018;16(2): 746-750.

[22]Mahmassani ZS, Son K, Pincu Y, et al. α7β1 Integrin regulation of gene transcription in skeletal muscle following an acute bout of eccentric exercise. Am J Phsiol Cell Physiol.2017;312(5): C638-C650.

[23]Wang D,Gao CQ,Chen RQ,et al.Focal adhesion kinase and paxillin promote migration and adhesion to fibronectin by swine skeletal muscle satellite cells.Oncotarget.2016;7(21): 30845-30854.

[24]Franchi MV,Ruoss S,Valdivieso P,et al.Regional regulation of focal adhesion kinase after concentric and eccentric loading is related to remodelling of human skeletal muscle.Acta Physiol(Oxf). 2018;223(3): e13056.

[25]Bataille L,Delon I,Da Ponte JP,et al.Downstream of identity genes: muscle-type-specific regulation of the fusion process.Dev Cell.2010; 19(2): 317-328.

[26]Hirth S,Buhler A,Buhrdel JB,et al.Paxillin and Focal Adhesion Kinase (FAK) Regulate Cardiac Contractility in the Zebrafish Heart. PloS One.2016;11(3): e0150323.

[27]Jacob AE,Amack JD,Turner CE.Paxillin Genes and Actomyosin Contractility Regulate Myotome Morphogenesis in Zebrafish. Dev Biol.2017;425(1): 70-84.

[28]Silva P,Mendoza P, Rivas S, et al. Hypoxia promotes Rab5 activation, leading to tumor cell migration, invasion and metastasis.Oncotarget. 2016;7(20):29548-29562.

[29]Janik ME,Litynska A,Vereecken P.Cell migration-the role of integrin glycosylation.Biochim Biophys Acta.2010;1800(6):545-555.

[30]Müller S,Djudjaj S,Lange J,et al.HIF stabilization inhibits renal epithelial cell migration and is associated with cytoskeletal alterations. Sci Rep.2018;8(1):9497.